Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-3-25
pubmed:abstractText
Genomic DNAs of various strains of Oxalobacter formigenes were subjected to restriction endonuclease fragment length polymorphism- and PCR-based amplification analyses with DNA probes and primers complementary to sequences within either the oxc gene, encoding oxalyl coenzyme A (oxalyl-CoA) decarboxylase, or the frc gene, encoding formyl-CoA transferase. Oligonucleotide probes based on nonconserved sequences of oxc or frc were able to divide O. formigenes strains into at least two groups, consistent with the current separation of O. formigenes strains into groups I and II on the basis of 16S rRNA sequence similarities and lipid content. In contrast, an oligonucleotide probe based on the conserved 5' end of oxc appeared to bind all group I and the majority of group II strains. PCR amplification of the oxc gene showed even greater sensitivity in detecting O. formigenes and provided support for further division of the strains into subgroups. In addition, these oligonucleotides failed to hybridize to or amplify PCR products from whole fecal DNA isolated from fresh stool samples from an individual not colonized with O. formigenes, indicating unique specificity. Thus, these DNA analyses permit both detection as well as classification of O. formigenes strains.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-13819869, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-14101969, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-2012090, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-3060247, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-3373343, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-3662516, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-3950772, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-3994481, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-7425628, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-7615704, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-8096218, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-8105989, http://linkedlifedata.com/resource/pubmed/commentcorrection/9003594-8157618
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
350-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Identification and classification of Oxalobacter formigenes strains by using oligonucleotide probes and primers.
pubmed:affiliation
Department of Pathology & Laboratory Medicine, University of Florida, Gainesville 32610, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't