Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-21
pubmed:databankReference
pubmed:abstractText
Three cDNA clones encoding NG,NG-dimethylarginine dimethylamino-hydrolase (EC 3.5.3.18) have been isolated from a rat kidney lambda gt11 library using a probe prepared by PCR on the basis of partially determined amino-acid sequences of the enzyme. The sequence analyses of the cDNAs established that the 3008 bp cDNA contains 855 bp open reading frame encoding 285 amino acids (M(r) 31414), 3'-flanking region (1722 bp) and 5'-flanking region (431 bp). The amino-acid sequences of all the peptides isolated from the purified enzyme were shown to be included in the deduced amino-acid sequence. Northern blotting analyses showed that the mRNA (4 kb) is ubiquitously expressed in various rat tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
1337
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney.
pubmed:affiliation
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
pubmed:publicationType
Journal Article