rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-2-21
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pubmed:databankReference |
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pubmed:abstractText |
Three cDNA clones encoding NG,NG-dimethylarginine dimethylamino-hydrolase (EC 3.5.3.18) have been isolated from a rat kidney lambda gt11 library using a probe prepared by PCR on the basis of partially determined amino-acid sequences of the enzyme. The sequence analyses of the cDNAs established that the 3008 bp cDNA contains 855 bp open reading frame encoding 285 amino acids (M(r) 31414), 3'-flanking region (1722 bp) and 5'-flanking region (431 bp). The amino-acid sequences of all the peptides isolated from the purified enzyme were shown to be included in the deduced amino-acid sequence. Northern blotting analyses showed that the mRNA (4 kb) is ubiquitously expressed in various rat tissues.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Jan
|
pubmed:issn |
0006-3002
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
4
|
pubmed:volume |
1337
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
6-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9003431-Amidohydrolases,
pubmed-meshheading:9003431-Amino Acid Sequence,
pubmed-meshheading:9003431-Animals,
pubmed-meshheading:9003431-Base Sequence,
pubmed-meshheading:9003431-Blotting, Northern,
pubmed-meshheading:9003431-Cloning, Molecular,
pubmed-meshheading:9003431-DNA, Complementary,
pubmed-meshheading:9003431-Hydrolases,
pubmed-meshheading:9003431-Kidney,
pubmed-meshheading:9003431-Molecular Sequence Data,
pubmed-meshheading:9003431-RNA, Messenger,
pubmed-meshheading:9003431-Rats,
pubmed-meshheading:9003431-Sequence Analysis, DNA
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney.
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pubmed:affiliation |
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
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pubmed:publicationType |
Journal Article
|