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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1997-2-11
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| pubmed:abstractText |
In tropical and subtropical regions of the world, allergens produced by Blomia tropicalis are an important cause of IgE-mediated sensitization among patients with asthma. We compared the relative importance of sensitization to the two mite species among asthma patients from Florida, Puerto Rico, and Brazil (n = 83), who were concurrently exposed to B. tropicalis and D. pteronyssinus, with patients from the United States and from the United Kingdom (n = 56) exposed to D. pteronyssinus. In addition, molecular cloning techniques were used to clone and express a major B. tropicalis allergen. There were significant differences between IgE antibody responses to B. tropicalis and D. pteronyssinus that were related to exposure: only 22% of patients exposed to both species had a high ratio (> 10) of IgE D. pteronyssinus:B. tropicalis, whereas 68% of patients exposed only to D. pteronyssinus had a ratio of > 10 (p < 0.001). A major 14-kD allergen (Blo t 5), cloned from a B. tropicalis cDNA library, showed 43% sequence homology to D. pteronyssinus Der p 5. Recombinant Blo t 5 produced in E. coli reacted with 45 to 69% of sera from B. tropicalis-allergic asthmatics and induced positive immediate skin tests at 10(-3) to 1 microg/ml. In vivo and in vitro comparisons of IgE responses to B. tropicalis, D. pteronyssinus, rBlo t 5, and rDer p 5, showed that B. tropicalis has unique allergens that cause specific IgE responses. The results suggest that B. tropicalis is an independent cause of sensitization and that use of recombinant Blo t 5 should lead to a better understanding of the role of B. tropicalis in causing asthma in tropical environments.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Allergens,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Dermatophagoides,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/allergen Blo t 5
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1073-449X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
155
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
343-50
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:9001334-Adolescent,
pubmed-meshheading:9001334-Adult,
pubmed-meshheading:9001334-Allergens,
pubmed-meshheading:9001334-Amino Acid Sequence,
pubmed-meshheading:9001334-Animals,
pubmed-meshheading:9001334-Antigens, Dermatophagoides,
pubmed-meshheading:9001334-Antigens, Plant,
pubmed-meshheading:9001334-Asthma,
pubmed-meshheading:9001334-Base Sequence,
pubmed-meshheading:9001334-Brazil,
pubmed-meshheading:9001334-Child,
pubmed-meshheading:9001334-Child, Preschool,
pubmed-meshheading:9001334-Cloning, Molecular,
pubmed-meshheading:9001334-Florida,
pubmed-meshheading:9001334-Glycoproteins,
pubmed-meshheading:9001334-Humans,
pubmed-meshheading:9001334-Immunoglobulin E,
pubmed-meshheading:9001334-Mites,
pubmed-meshheading:9001334-Molecular Sequence Data,
pubmed-meshheading:9001334-Puerto Rico,
pubmed-meshheading:9001334-Recombinant Proteins,
pubmed-meshheading:9001334-Sequence Homology,
pubmed-meshheading:9001334-Skin Tests,
pubmed-meshheading:9001334-Tropical Climate
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| pubmed:year |
1997
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| pubmed:articleTitle |
Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5.
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| pubmed:affiliation |
Asthma and Allergic Diseases Center, Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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