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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1997-3-26
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pubmed:abstractText |
The expression of activation antigens, namely CD25, CD69, CD71, and HLA-DR on T cells from 15 healthy individuals stimulated with different mitogens and specific antigens was evaluated by immunofluorescence assay and flow cytometric analysis and compared with cell proliferation as a function of [3H]thymidine incorporation. CD69 was the earliest expressed antigen on stimulated cells, while HLA-DR was the latest. Regardless of the stimulus used, lymphocytes expressing CD25 and CD71 were always more numerous than cells expressing CD69 and HLA-DR. Variations in the proportion of CD4+ and CD8+ T cells expressing each activation marker were observed with different antigenic stimuli. The expression of each activation marker showed overall agreement with the [3H]thymidine incorporation assay in discriminating between positive and negative immune response. However, no correlation was observed between the percentage of CD25-, CD69-, CD71-, and HLA-DR-positive T cells and the amount of [3H]thymidine incorporation. Moreover, low doses of mitogens and antigens as well as short time of stimulation were sufficient to induce T cells to express activation antigens but not to proliferate. Our data show that results obtained by flow cytometry and [3H]thymidine incorporation may differ qualitatively, at least under certain conditions; this suggests that the 2 assays are complementary, and when combined, may gives a clearer understanding of events leading to efficient cell-mediated immune response.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0196-4763
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9000587-Amino Acid Sequence,
pubmed-meshheading:9000587-Antigens, CD,
pubmed-meshheading:9000587-Cell Division,
pubmed-meshheading:9000587-Cells, Cultured,
pubmed-meshheading:9000587-DNA,
pubmed-meshheading:9000587-Flow Cytometry,
pubmed-meshheading:9000587-HLA-DR Antigens,
pubmed-meshheading:9000587-Humans,
pubmed-meshheading:9000587-Leukocytes, Mononuclear,
pubmed-meshheading:9000587-Lymphocyte Activation,
pubmed-meshheading:9000587-Lymphocyte Subsets,
pubmed-meshheading:9000587-Mitogens,
pubmed-meshheading:9000587-Molecular Sequence Data,
pubmed-meshheading:9000587-T-Lymphocytes,
pubmed-meshheading:9000587-Thymidine
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pubmed:year |
1997
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pubmed:articleTitle |
Flow cytometric analysis of activation markers on stimulated T cells and their correlation with cell proliferation.
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pubmed:affiliation |
Institute of Microbiology, University of Brescia Medical School, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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