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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6 Pt 2
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pubmed:dateCreated |
1997-2-20
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pubmed:abstractText |
Contractile arrest of cardiac myocytes results in increased abundance of alpha-myosin heavy chain (MHC) mRNA but decreased alpha-MHC protein content. Our aim is to determine the posttranscriptional mechanisms regulating alpha-MHC mRNA-protein uncoupling in cultured neonatal rat hearts during altered contractile activity. Spontaneously contracting myocytes were arrested by the use of verapamil (10 mumol/l; a Ca(2+)-channel blocker) or by 2,3-butanedione monoxime (5 mmol/l; a cross-bridge inhibitor). Inhibition of transcription with amanitin (0.5 mumol/l) decreased the alpha-MHC mRNA in normally beating myocytes to a minimal baseline. However, the alpha-MHC mRNA did not fall this low in amanitin-treated nonbeating myocytes. Concurrently, the alpha-MHC mRNA shifted toward a heavier polysome complex when beating was blocked. Together, these data suggest that contractile arrest regulates alpha-MHC mRNA abundance posttranscriptionally by stabilizing the message at the elongation phase of translation. These posttranscriptional regulatory steps are dependent on beating itself and are independent of Ca2+ entry.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Diacetyl,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Myosins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/diacetylmonoxime
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H2584-90
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8997319-Animals,
pubmed-meshheading:8997319-Cells, Cultured,
pubmed-meshheading:8997319-Diacetyl,
pubmed-meshheading:8997319-Drug Stability,
pubmed-meshheading:8997319-Myocardial Contraction,
pubmed-meshheading:8997319-Myocardium,
pubmed-meshheading:8997319-Myosin Heavy Chains,
pubmed-meshheading:8997319-Myosins,
pubmed-meshheading:8997319-Polyribosomes,
pubmed-meshheading:8997319-Protein Processing, Post-Translational,
pubmed-meshheading:8997319-RNA, Messenger,
pubmed-meshheading:8997319-Rats,
pubmed-meshheading:8997319-Rats, Sprague-Dawley,
pubmed-meshheading:8997319-Tissue Distribution
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pubmed:year |
1996
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pubmed:articleTitle |
Beating affects the posttranscriptional regulation of alpha-myosin mRNA in cardiac cultures.
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pubmed:affiliation |
Department of Physiology and Biophysics, University of Illinois College of Medicine at Chicago 60612-7342, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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