Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-12
pubmed:abstractText
Insulin receptor substrate 1 (IRS-1), and its structural relative IRS-2, are both phosphorylated on tyrosine following treatment of cells with interleukin-4 (IL-4) and insulin. We have investigated whether both IRS-1 and IRS-2 are expressed in murine lymphohemopoietic cells. T and B lymphocytes and macrophages from primary cultures expressed only IRS-2, which became phosphorylated on tyrosine following stimulation with both IL-4 and insulin. Likewise, the murine myeloid cell line FD-5 expressed only IRS-2, which was tyrosine phosphorylated in response to IL-4 and insulin, as well as interleukin-3 and granulocyte-macrophage colony stimulating factor. Neither IRS-1 nor IRS-2 were expressed at detectable levels in primary bone marrow mast cells although these cells do respond to IL-4. Moreover, a factor-dependent lymphocyte cell line, CT.4S, which grows continuously in IL-4, did not express detectable levels of IRS-1 or IRS-2. IRS-2 from FD-5 cells stimulated with either IL-4 or insulin bound to glutathione S-transferase fusion proteins of the p85 subunit of phosphoinositol 3'-kinase, Grb2, and Syp, paralleling reported associations of IRS-1 with these molecules and indicating phosphorylation of the corresponding residues on IRS-2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing, http://linkedlifedata.com/resource/pubmed/chemical/Avian Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cytokines, http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/GRB2 Adaptor Protein, http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage..., http://linkedlifedata.com/resource/pubmed/chemical/Grb2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Insulin Receptor Substrate Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/Irs1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Irs2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ptpn11 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Ptpn6 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine, http://linkedlifedata.com/resource/pubmed/chemical/cytoskeletal p85 protein, chicken
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1377-81
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8995447-Adaptor Proteins, Signal Transducing, pubmed-meshheading:8995447-Animals, pubmed-meshheading:8995447-Avian Proteins, pubmed-meshheading:8995447-Cytokines, pubmed-meshheading:8995447-Cytoskeletal Proteins, pubmed-meshheading:8995447-GRB2 Adaptor Protein, pubmed-meshheading:8995447-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:8995447-Hematopoietic Stem Cells, pubmed-meshheading:8995447-Insulin, pubmed-meshheading:8995447-Insulin Receptor Substrate Proteins, pubmed-meshheading:8995447-Interleukin-3, pubmed-meshheading:8995447-Interleukin-4, pubmed-meshheading:8995447-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:8995447-Lymphocytes, pubmed-meshheading:8995447-Mice, pubmed-meshheading:8995447-Molecular Weight, pubmed-meshheading:8995447-Phosphoproteins, pubmed-meshheading:8995447-Phosphorylation, pubmed-meshheading:8995447-Protein Tyrosine Phosphatase, Non-Receptor Type 11, pubmed-meshheading:8995447-Protein Tyrosine Phosphatase, Non-Receptor Type 6, pubmed-meshheading:8995447-Protein Tyrosine Phosphatases, pubmed-meshheading:8995447-Proteins, pubmed-meshheading:8995447-Receptor, Epidermal Growth Factor, pubmed-meshheading:8995447-Tyrosine
pubmed:year
1997
pubmed:articleTitle
Insulin receptor substrate-2 is the major 170-kDa protein phosphorylated on tyrosine in response to cytokines in murine lymphohemopoietic cells.
pubmed:affiliation
The Biomedical Research Centre, University of British Columbia, Vancouver, Canada. prsmjw@bath.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't