Linked Life Data

8995371

Source:http://linkedlifedata.com/resource/pubmed/id/8995371

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-12
pubmed:databankReference
pubmed:abstractText
Dentin is the major mineralized extracellular matrix of the tooth. The organic components of dentin consist of type I collagen (90%) with 10% noncollagenous proteins, which are also components of bone. Two dentin proteins, dentin sialoprotein and dentin phosphoprotein, have been shown to be tooth-specific being expressed mostly by odontoblast cells. In this study, we screened a mouse molar tooth library for dentin sialoprotein and dentin phosphoprotein cDNA clones. Analysis of the clones resulted in characterization of a 4420-nucleotide cDNA that contained a 940-amino acid open reading frame. The signal peptide and NH2-terminal sequence was 75% homologous to the cDNA sequence of rat dentin sialoprotein. The continued open reading frame, however, contained a RGD sequence followed by a region of repeated aspartic acid and serine residues. This portion of the protein codes for amino acid sequence consistent with that of dentin phosphoprotein. The noncoding region contains three potential polyadenylation signals, two of which were shown to be utilized. Northern blot analysis indicated the presence of two major transcripts of 4.4 and 2.2 kilobases in odontoblasts. Chromosomal mapping localized the gene to human chromosome 4. These data suggest that the previously identified dentin extracellular matrix proteins, dentin sialoprotein and dentin phosphoprotein, are expressed as a single cDNA transcript coding for a protein that is specifically cleaved into two smaller polypeptides with unique physical-chemical characteristics. Therefore, we propose that the gene be named dentin sialophosphoprotein. The location of the human dentin sialophosphoprotein gene on chromosome 4 suggests that this gene may be a strong candidate gene for the genetic disease dentinogenesis imperfecta type II.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
835-42
pubmed:dateRevised
2010-2-5
pubmed:meshHeading
pubmed-meshheading:8995371-Amino Acid Sequence, pubmed-meshheading:8995371-Animals, pubmed-meshheading:8995371-Base Sequence, pubmed-meshheading:8995371-Blotting, Northern, pubmed-meshheading:8995371-Chromosomes, Human, Pair 4, pubmed-meshheading:8995371-DNA, pubmed-meshheading:8995371-Extracellular Matrix Proteins, pubmed-meshheading:8995371-Frameshift Mutation, pubmed-meshheading:8995371-Humans, pubmed-meshheading:8995371-Mice, pubmed-meshheading:8995371-Molecular Sequence Data, pubmed-meshheading:8995371-Phosphoproteins, pubmed-meshheading:8995371-Polymerase Chain Reaction, pubmed-meshheading:8995371-Protein Precursors, pubmed-meshheading:8995371-Protein Processing, Post-Translational, pubmed-meshheading:8995371-Rats, pubmed-meshheading:8995371-Sequence Alignment, pubmed-meshheading:8995371-Sialoglycoproteins, pubmed-meshheading:8995371-Species Specificity
pubmed:year
1997
pubmed:articleTitle
Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. Dentin phosphoprotein DNA sequence determination.
pubmed:affiliation
University of Texas Health Science Center at San Antonio, Dental School, Department of Pediatric Dentistry, 78284-7888, USA. MacDougall@UTHSCSA.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.