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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-1-31
|
| pubmed:abstractText |
In the A/J strain of mice, urethane (ethyl carbamate) induces lung hyperplasia, adenoma, and adenocarcinoma in a time-dependent manner. These distinct morphological stages may correlate with sequential molecular genetic changes in this mouse model. To test this hypothesis, we investigated the presence of mutations involving Ki-ras and p53 in urethane-induced lung lesions in A/J mice at early and late stages of tumorigenesis. We precisely microdissected 40 lung lesions from paraffin-embedded sections. Ki-ras mutations around codon 61 and p53 mutations in exons 5-8 were identified by polymerase chain reaction-single-strand conformation polymorphism and DNA sequencing techniques. In 29 early-stage lung lesions classified as hyperplasias (seven) or adenomas (22), we observed 19 Ki-ras mutations (66%), including three silent mutations and one double mutation at different codons, and one silent p53 mutation (3.5%). In 11 late-stage adenomas, we identified nine activating Ki-ras mutations (82%) and four missense p53 mutations (36%). These results indicate that Ki-ras mutations arise early, whereas p53 mutations occur relatively late during the benign stages of urethane-induced lung carcinogenesis in A/J mice.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0899-1987
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
217-23
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8989915-Adenocarcinoma,
pubmed-meshheading:8989915-Adenoma,
pubmed-meshheading:8989915-Animals,
pubmed-meshheading:8989915-Base Sequence,
pubmed-meshheading:8989915-Carcinogens,
pubmed-meshheading:8989915-Disease Progression,
pubmed-meshheading:8989915-Exons,
pubmed-meshheading:8989915-Genes, p53,
pubmed-meshheading:8989915-Genes, ras,
pubmed-meshheading:8989915-Hyperplasia,
pubmed-meshheading:8989915-Lung Neoplasms,
pubmed-meshheading:8989915-Mice,
pubmed-meshheading:8989915-Mice, Inbred A,
pubmed-meshheading:8989915-Molecular Sequence Data,
pubmed-meshheading:8989915-Mutation,
pubmed-meshheading:8989915-Precancerous Conditions,
pubmed-meshheading:8989915-Time Factors,
pubmed-meshheading:8989915-Urethane
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Ki-ras and p53 mutations are early and late events, respectively, in urethane-induced pulmonary carcinogenesis in A/J mice.
|
| pubmed:affiliation |
Department of Thoracic and Cardiovascular Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|