Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-7-7
|
| pubmed:abstractText |
The primary cytotoxicity of Staphylococcal enterotoxin B (SEB)-stimulated PBMC and separated CD8+ and CD4+ T cells against Burkitt's lymphoma target cells has been characterized by applying two stimulation protocols. In the bulk protocol, the PBMC were stimulated with 100 ng/mL SEB for 3 days before separation to CD4+ and CD8+ T subsets. In the direct protocol, the separated CD4+ and CD8+ T cells were stimulated with 100 ng SEB preabsorbed to mitomycin C (MMC)-treated APC. Comparison of the results of the two different protocols revealed the following differences: (i) PBMC in the direct protocol provided greater cytolytic activity than in the bulk protocol; and (ii) the CD4+ T cells acquired cytotoxicity only in the direct protocol. Unexpectedly, the superantigen-dependent cellular cytotoxicity (SDCC) of SEB-stimulated cells was not dominant compared with the basal cytotoxicity. The classical NK target, K-562 was also sensitive to SEB-augmented cytotoxicity. The parallel stimulation with IL-2 and SEB caused a similar extent of cytotoxicity enhancement against both types of target cells. However, the cyclosporin A (CSA) inhibited only the SEB-induced cytotoxicity. The results suggest that SEB-induced PBMC acquire mainly a LAK-like cytotoxicity, as a consequence of newly produced lymphokines. This observation might propose a different approach in pathological studies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0818-9641
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
483-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8989585-Antigen-Presenting Cells,
pubmed-meshheading:8989585-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8989585-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8989585-Cell Separation,
pubmed-meshheading:8989585-Cytotoxicity, Immunologic,
pubmed-meshheading:8989585-Enterotoxins,
pubmed-meshheading:8989585-Humans,
pubmed-meshheading:8989585-Interleukin-2,
pubmed-meshheading:8989585-Leukocytes, Mononuclear,
pubmed-meshheading:8989585-Lymphocyte Activation,
pubmed-meshheading:8989585-Staphylococcus aureus,
pubmed-meshheading:8989585-Superantigens,
pubmed-meshheading:8989585-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Primary cytotoxicity of SEB-activated human PBMC and separated CD8+ and CD4+ T lymphocytes elicited by two different stimulation protocols.
|
| pubmed:affiliation |
National Medical Children's Medical Research Center, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|