Linked Life Data

8987448

Source:http://linkedlifedata.com/resource/pubmed/id/8987448

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-3
pubmed:abstractText
A new and very sensitive antigen detection technique, immuno-polymerase chain reaction (immuno-PCR), was developed. This method is basically similar to the enzyme-linked immunosorbent assay which detects an antigen-antibody reaction, but instead of an enzyme being conjugated to an antibody, a DNA fragment is used and this DNA can be amplified by PCR. We applied this method to the detection of the fish pathogen, Pasteurella piscicida, in naturally infected yellowtail. Using immuno-PCR, 3.4 cfu ml-1 of bacteria could be detected. In comparison, ELISA detected only 3.4 x 10(4) cfu ml-1. Immuno-PCR is a powerful method for detection of pathogens in host tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0266-8254
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-3
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Detection of bacterial antigens using immuno-PCR.
pubmed:affiliation
Faculty of Agriculture, Miyazaki University, Japan.
pubmed:publicationType
Journal Article, Comparative Study