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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1997-5-23
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pubmed:abstractText |
Attempts were undertaken to develop cyclic beta-casomorphin-5 analogs with improved opioid activity profiles by deletion of the glycine residue in position 5, leading to analogs structurally related to the opioid peptide morphiceptin (H-Tyr-Pro-Phe-Pro-NH2). The tetrapeptide sequence Boc-Tyr(tBu)-D-Xaa-Phe-Yaa-OH (Xaa = Lys, Orn, A2bu; Yaa = Pro in L- or D-configuration) was used to study the influence of ring size and chirality on the yield of cyclization between the omega-amino group of Xaa and the C-terminal carboxyl group. In all cases the cyclization reaction was performed under identical experimental conditions to allow a direct comparison with regard to yield and homogeneity. The reaction products were purified by crystallization and liquid chromatography, and were characterized by HPLC, TLC, electrospray mass spectrometry and 1H-NMR spectroscopy. In none of the reactions performed with the cyclization precursors containing proline in the L-configuration could a cyclic monomer be detected, and the cyclodimer (7-9) was the exclusive product in each case. Cyclodimerization was also the favored reaction in the attempted formation of the 11-membered ring of the cyclic [D-A2bu2, D-Pro4]-morphiceptin analog 12, since only traces of the monomer were found. In the case of both the [D-Lys2, D-Pro4]-analog 10 and the [D-Orn2, D-Pro4]-analog 11, the cyclomonomer/cyclodimer ratio was about 80:20. The cyclic monomers 10 and 11 showed high opioid activity in the mu-receptor-representative guinea pig ileum assay (IC50 = 2-5 nM) and in the delta-receptor representative mouse vas deferens assay (IC50 = 50-60 nM), whereas the potency of the cyclodimers was 2-3 orders of magnitude lower in both in vitro bioassays.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics,
http://linkedlifedata.com/resource/pubmed/chemical/Endorphins,
http://linkedlifedata.com/resource/pubmed/chemical/Opioid Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides, Cyclic,
http://linkedlifedata.com/resource/pubmed/chemical/beta-casomorphin 5,
http://linkedlifedata.com/resource/pubmed/chemical/morphiceptin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0367-8377
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
495-502
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8985782-Analgesics,
pubmed-meshheading:8985782-Animals,
pubmed-meshheading:8985782-Endorphins,
pubmed-meshheading:8985782-Guinea Pigs,
pubmed-meshheading:8985782-Ileum,
pubmed-meshheading:8985782-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8985782-Male,
pubmed-meshheading:8985782-Mass Spectrometry,
pubmed-meshheading:8985782-Mice,
pubmed-meshheading:8985782-Muscle Contraction,
pubmed-meshheading:8985782-Opioid Peptides,
pubmed-meshheading:8985782-Peptide Fragments,
pubmed-meshheading:8985782-Peptides, Cyclic,
pubmed-meshheading:8985782-Structure-Activity Relationship,
pubmed-meshheading:8985782-Vas Deferens
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pubmed:year |
1996
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pubmed:articleTitle |
Cyclic morphiceptin analogs: cyclization studies and opioid activities in vitro.
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pubmed:affiliation |
Department of Drug Biochemistry, Hans-Knoell-Institute of Natural Product Research Jena, Halle, Saale, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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