| pubmed-article:8985123 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C0136072 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:8985123 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:8985123 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:8985123 | pubmed:dateCreated | 1997-1-31 | lld:pubmed |
| pubmed-article:8985123 | pubmed:abstractText | The gene encoding rat peptidylglycine alpha-amidating monooxygenase (PAM) contains 26 protein-coding exons. We identified two non-overlapping genomic clones encoding the 5' untranslated region (UTR) of the PAM gene. Exon 1 has 69 nucleotides flanked by perfect splice acceptor and donor sites, with a TATA motif 25 nucleotides upstream. Exon 0 lacks TATA or CAAT motifs and is embedded in a G + C-rich 800-nucleotide CpG island. The major products identified by RNase protection initiated in exon 0; only a minority of mRNAs initiated in exon 1. 5'-rapid amplification of cDNA ends (RACE) identified the same major transcriptional start sites in exon 0 in the atrium and neurointermediate pituitary. The 2.0-kb fragment upstream of exon 0 and the 1.3-kb fragment upstream of exon 1 were placed upstream of a luciferase-based reporter gene in both sense and antisense orientations. Expression of luciferase was observed in neuroendocrine and nonneuroendocrine cells with both sense constructs. A 0.2-kb fragment of the exon 0 PAM promoter containing multiple GC box elements supported expression of luciferase activity in all cell types. Expression of reporter genes in cells that do not normally express PAM suggests a need for more upstream or intronic information, a role for methylation, or a need for chromatin scaffolding for tissue-specific expression of the endogenous gene. | lld:pubmed |
| pubmed-article:8985123 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8985123 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8985123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8985123 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8985123 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8985123 | pubmed:issn | 1044-5498 | lld:pubmed |
| pubmed-article:8985123 | pubmed:author | pubmed-author:EipperB ABA | lld:pubmed |
| pubmed-article:8985123 | pubmed:author | pubmed-author:MainsR ERE | lld:pubmed |
| pubmed-article:8985123 | pubmed:author | pubmed-author:HandT ATA | lld:pubmed |
| pubmed-article:8985123 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8985123 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:8985123 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8985123 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8985123 | pubmed:pagination | 1093-104 | lld:pubmed |
| pubmed-article:8985123 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:meshHeading | pubmed-meshheading:8985123-... | lld:pubmed |
| pubmed-article:8985123 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8985123 | pubmed:articleTitle | Identification of the promoter for the gene encoding the bifunctional enzyme, peptidylglycine alpha-amidating monooxygenase. | lld:pubmed |
| pubmed-article:8985123 | pubmed:affiliation | Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. | lld:pubmed |
| pubmed-article:8985123 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8985123 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| entrez-gene:25508 | entrezgene:pubmed | pubmed-article:8985123 | lld:entrezgene |
