Linked Life Data

8983291

Source:http://linkedlifedata.com/resource/pubmed/id/8983291

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-1-7
pubmed:abstractText
Previous studies from our laboratory have shown that HT29 cells selected by adaptation to methotrexate (HT29-MTX) express mature mucins that differ in their immunoreactivity to antibodies against gastric mucin and in the level of one of two major gastric mucin MUC5AC (MUC5) mRNA compared with parental HT29 cells. In this study, we examined the expression of another major gastric mucin, MUC6 mRNA, as well as that of MUC2, -3 and -5 mRNAs in HT29-MTX cells. We also examined their relationship to mucin-related antigen expression and biological properties of the cells such as adhesion to matrigel and E-selectin and in vitro invasiveness, liver colonising activity and degree of differentiation of nude mouse xenograft. Slot blot and Northern analysis revealed markedly increased levels of MUC5 mRNA but no change in MUC6 mRNA level in HT29-MTX cells compared with parental HT29 cells which express barely detectable levels of MUC6 mRNA. A nuclear run-on study showed that MUC5 mRNA was up-regulated at the transcriptional level. The marked increase in MUC5 mRNA was associated with a significant increase in the expression of human gastric mucin and apomucin antigens in HT29-MTX cells. When the adhesive capacity of two cell lines was compared, HT29-MTX cells showed significantly lower adhesion to E-selectin consistent with their lower expression of sialyl Le(x) and sialyl Le(a) antigens compared with HT29 cells. HT29-MTX cells also showed lower adhesive capacity to matrigel than HT29 cells. Interestingly, HT29-MTX cells exhibited significantly decreased liver colonisation capacity in nude mice following splenic vein injection. Furthermore, nude mouse xenograft tumours produced by HT29-MTX cells exhibited a significantly greater degree of differentiation, consisting of mucin-secreting glands than those produced by HT29 cells. In conclusion, these results indicate a shift of predominantly colonic-type mucins to the gastric type, specifically the surface epithelial cell type (MUC5) but not the mucous neck cell or antral gland type (MUC6) in HT29-MTX cells and strongly suggest that altered regulation of mucin genes and the degree of differentiation in cancer cells may be responsible for the altered biological behaviour of these cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0959-8049
pubmed:author
pubmed:issnType
Print
pubmed:volume
32A
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1788-96
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8983291-Animals, pubmed-meshheading:8983291-Blotting, Northern, pubmed-meshheading:8983291-Collagen, pubmed-meshheading:8983291-Drug Combinations, pubmed-meshheading:8983291-Drug Resistance, Neoplasm, pubmed-meshheading:8983291-E-Selectin, pubmed-meshheading:8983291-HT29 Cells, pubmed-meshheading:8983291-Humans, pubmed-meshheading:8983291-Immunoblotting, pubmed-meshheading:8983291-Laminin, pubmed-meshheading:8983291-Methotrexate, pubmed-meshheading:8983291-Mice, pubmed-meshheading:8983291-Mice, Nude, pubmed-meshheading:8983291-Mucins, pubmed-meshheading:8983291-Neoplasm Transplantation, pubmed-meshheading:8983291-Proteoglycans, pubmed-meshheading:8983291-RNA, Messenger, pubmed-meshheading:8983291-RNA, Neoplasm, pubmed-meshheading:8983291-Transcription, Genetic, pubmed-meshheading:8983291-Transplantation, Heterologous
pubmed:year
1996
pubmed:articleTitle
Alteration in mucin gene expression and biological properties of HT29 colon cancer cell subpopulations.
pubmed:affiliation
Kagoshima University School of Medicine, Kagoshima City, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.