Linked Life Data

8982730

Source:http://linkedlifedata.com/resource/pubmed/id/8982730

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-4-2
pubmed:abstractText
Renal mesangial cells exposed to inflammatory cytokines produce high concentrations of nitric oxide (NO) which may exert cytotoxic actions. We report here that glomerular mesangial cells, endothelial cells and epithelial cells in culture are themselves targets for NO and undergo apoptotic cell death upon exposure to high concentrations of NO. NO generated from different NO-releasing compounds as well as NO-saturated solution induce apoptosis in all three cell types as demonstrated by internucleosomal DNA fragmentation, an enrichment of cytosolic DNA/histone complexes, an increasing number of cellular 3'-OH-fragmented DNA ends and typical nuclear chromatin condensation. Induction of apoptosis was found to be dependent on protein synthesis and is preceded by expression of the tumour suppressor gene product p53 in mesangial cells. Induction of inducible NO synthase in mesangial cells by interleukin-1 beta leads to excessive formation of NO by the cells as measured by nitrite production. However, there was no evidence for apoptotic changes in mesangial cells triggered by endogenously produced NO. Co-cultures of glomerular endothelial or epithelial cells with interleukin-1 beta-activated mesangial cells expressing inducible NO synthase do not show apoptotic alterations in endothelial or epithelial cells. Moreover, preincubation of mesangial cells with interleukin-1 beta protects the cells from apoptosis induced by subsequent addition of exogenous NO thus suggesting that interleukin-1 beta not only triggers the expression of inducible NO synthase and massive NO formation but simultaneously stimulates a protecting principle in the cells. In summary, these results suggest that exogenous NO can induce apoptosis in all three types of intrinsic glomerular cells. However, whether endogenously produced NO can fulfil this function critically depends on a balance between a yet to be defined protective mechanism and inducible NO synthase expression in mesangial cells in response to interleukin-1 beta and eventually other inflammatory cytokines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
317
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-49
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8982730-Animals, pubmed-meshheading:8982730-Apoptosis, pubmed-meshheading:8982730-Cell Nucleus, pubmed-meshheading:8982730-DNA Fragmentation, pubmed-meshheading:8982730-Electrophoresis, Agar Gel, pubmed-meshheading:8982730-Enzyme Inhibitors, pubmed-meshheading:8982730-Epithelial Cells, pubmed-meshheading:8982730-Epithelium, pubmed-meshheading:8982730-Female, pubmed-meshheading:8982730-Glomerular Mesangium, pubmed-meshheading:8982730-Immunohistochemistry, pubmed-meshheading:8982730-Interleukin-1, pubmed-meshheading:8982730-Male, pubmed-meshheading:8982730-Nitric Oxide, pubmed-meshheading:8982730-Penicillamine, pubmed-meshheading:8982730-Rats, pubmed-meshheading:8982730-Rats, Sprague-Dawley, pubmed-meshheading:8982730-Rats, Wistar, pubmed-meshheading:8982730-S-Nitroso-N-Acetylpenicillamine, pubmed-meshheading:8982730-Sodium-Potassium-Exchanging ATPase, pubmed-meshheading:8982730-Tumor Suppressor Protein p53, pubmed-meshheading:8982730-Uridine Triphosphate, pubmed-meshheading:8982730-Urothelium
pubmed:year
1996
pubmed:articleTitle
Nitric oxide donors induce apoptosis in glomerular mesangial cells, epithelial cells and endothelial cells.
pubmed:affiliation
Department of Pharmacology, Biozentrum, University of Basel, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't