Linked Life Data

8981352

Source:http://linkedlifedata.com/resource/pubmed/id/8981352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-3-14
pubmed:abstractText
A PCR method for detection of Yersinia pestis-virulence determinants by the use of multiplex primers was developed. Four pairs of oligonucleotide primers were designed from each gene of three kinds of virulent plasmids and a chromosomal DNA; 60-Md plasmid-located gene (caf1)encoding Y.pestis-specific capsular antigen fraction 1, a Y.pestis-specific region of a yopM gene encoded on 42-Md virulent plasmid, a plasminogen activator gene (pla) encoded on Y.pestis-specific 7-Md plasmid and an invasin protein gene (inv) encoded on chromosomal DNA. This multiplex-primer system was specific for the detection of Y.pestis among pathogenic Yersinia species and other enterobacteriaceae having antigens common to Y.pestis. Since this method is simple and safe, it will be useful to identify and confirm Y.pestis in cases of emergency and for the surveillance of epidemics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0385-5600
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
773-5
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Detection and identification of Yersinia pestis by polymerase chain reaction (PCR) using multiplex primers.
pubmed:affiliation
Department of Bacteriology, National Institute of Health, Tokyo, Japan.
pubmed:publicationType
Journal Article