Linked Life Data

8981309

Source:http://linkedlifedata.com/resource/pubmed/id/8981309

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-3-20
pubmed:abstractText
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder causing marked pathology in the motoneuron system. The pathophysiology of the selective degeneration of motor neurons in the disease is as yet unknown, but evidence suggests that excitotoxic mechanisms might be involved. The present study was undertaken to determine whether defects in neurotransmitter receptors are involved in the disease, analyzing uniformly sampled specimens from neocortex and motorcortex. The binding to benzodiazepine, muscarinic cholinergic, and NMDA receptors in ALS brains was compared to that in control brains, using a single radioligand concentration of [3H]Ro 15-1788, [3H]QNB and [3H]MK-801. The benzodiazepine and the muscarinic cholinergic receptor binding was unaffected in any cortical region from the ALS subjects compared to controls. NMDA receptor binding labeled by [3H]MK-801 was significantly increased in several neocortical regions in the ALS group compared to the control group. Scatchard analysis of [3H]MK-801 binding in frontal cortex revealed a single binding site with an unaltered maximal binding capacity but an increased binding affinity of the site in the ALS group compared to the controls. The generalized alteration in the affinity of the binding site for [3H]MK-801 in the ALS cortex may indicate a modification of the NMDA receptor due to different sensitivity for endogenous modulators or to a different subunit composition of the NMDA receptor in ALS with altered functional properties. These findings may reflect a pathophysiological phenomenon in ALS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-510X
pubmed:author
pubmed:issnType
Print
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
121-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8981309-Aged, pubmed-meshheading:8981309-Amyotrophic Lateral Sclerosis, pubmed-meshheading:8981309-Binding Sites, pubmed-meshheading:8981309-Brain Chemistry, pubmed-meshheading:8981309-Dizocilpine Maleate, pubmed-meshheading:8981309-Excitatory Amino Acid Antagonists, pubmed-meshheading:8981309-Female, pubmed-meshheading:8981309-Flumazenil, pubmed-meshheading:8981309-GABA Modulators, pubmed-meshheading:8981309-Humans, pubmed-meshheading:8981309-Male, pubmed-meshheading:8981309-Middle Aged, pubmed-meshheading:8981309-Motor Cortex, pubmed-meshheading:8981309-Muscarinic Antagonists, pubmed-meshheading:8981309-Quinuclidinyl Benzilate, pubmed-meshheading:8981309-Radioligand Assay, pubmed-meshheading:8981309-Receptors, GABA-A, pubmed-meshheading:8981309-Receptors, Muscarinic, pubmed-meshheading:8981309-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:8981309-Tritium
pubmed:year
1996
pubmed:articleTitle
Muscarinic, N-methyl-D-aspartate (NMDA) and benzodiazepine receptor binding sites in cortical membranes from amyotrophic lateral sclerosis patients.
pubmed:affiliation
Department of Biochemistry, Research Institute of Biological Psychiatry, St. Hans Hospital, Roskilde, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't