Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1997-1-30
|
pubmed:abstractText |
Although stimulation of freshly isolated murine spleen cells with anti-CD3 mAb or Con A failed to generate IL-12 production, the same cell preparations depleted of B cells produced IL-12. Addition of normal B cells inhibited IL-12 production in a cell number-dependent manner. IL-12 production was dependent on the presence of CD4+, but not of CD8+, T cells, and inhibited by addition of anti-CD40 ligand (CD40L) mAb. Anti-CD3 or Con A stimulation induced CD40L expression only on CD4+ T cells, which was inhibited in the presence of B cells. IL-12 production was also induced by interactions between CD40L-transfected Chinese hamster ovary cells and splenocytes depleted of T and B cells, but not of APC, indicating CD40L-induced IL-12 production by APC. The involvement of CD40 molecules was examined by comparing the ability of cells from CD40-deficient (CD40 -/-) and wild-type mice (CD40 +/+) to produce IL-12. Spleen cells from CD40 -/- and CD40 +/+ mice produced comparable amounts of IL-12 in response to bacterial stimuli. However, the B cell-depleted fraction from CD40 -/- mice failed to produce IL-12 when stimulated with anti-CD3 or Con A or when cocultured with CD40L-expressing Chinese hamster ovary cells. These results indicate that CD40L expressed on activated T cells induces APC to produce IL-12 through CD40/CD40L interaction, but this pathway is competitively inhibited by CD40+ B cells incapable of producing IL-12 upon stimulation with CD40L. Thus, this might represent a novel mechanism underlying the regulation of cell-mediated and humoral immunity.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0022-1767
|
pubmed:author |
pubmed-author:FujiwaraHH,
pubmed-author:HamaokaTT,
pubmed-author:KanekoYY,
pubmed-author:KikutaniHH,
pubmed-author:KishimotoTT,
pubmed-author:KobayashiMM,
pubmed-author:LIT JTJ,
pubmed-author:MaruoSS,
pubmed-author:Oh-horaMM,
pubmed-author:OkumuraKK,
pubmed-author:TrinchieriGG,
pubmed-author:WooV LVL,
pubmed-author:WysockaMM,
pubmed-author:YagitaHH
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
158
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
120-6
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:8977182-Animals,
pubmed-meshheading:8977182-Antibodies, Monoclonal,
pubmed-meshheading:8977182-Antigen-Presenting Cells,
pubmed-meshheading:8977182-Antigens, CD3,
pubmed-meshheading:8977182-B-Lymphocytes,
pubmed-meshheading:8977182-CD40 Ligand,
pubmed-meshheading:8977182-Cell Adhesion,
pubmed-meshheading:8977182-Coculture Techniques,
pubmed-meshheading:8977182-Concanavalin A,
pubmed-meshheading:8977182-Female,
pubmed-meshheading:8977182-Interleukin-12,
pubmed-meshheading:8977182-Ligands,
pubmed-meshheading:8977182-Membrane Glycoproteins,
pubmed-meshheading:8977182-Mice,
pubmed-meshheading:8977182-Mice, Inbred C57BL,
pubmed-meshheading:8977182-Mice, Mutant Strains,
pubmed-meshheading:8977182-T-Lymphocytes
|
pubmed:year |
1997
|
pubmed:articleTitle |
B cells regulate CD40 ligand-induced IL-12 production in antigen-presenting cells (APC) during T cell/APC interactions.
|
pubmed:affiliation |
Biomedical Research Center, Osaka University Medical School, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|