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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-1-30
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pubmed:abstractText |
To study the role of CD8 beta in T cell function, we derived a CD8 alpha/beta-(CD8-/-) T cell hybridoma of the H-2Kd-restricted N9 cytotoxic T lymphocyte clone specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260. This hybridoma was transfected either with CD8 alpha alone or together with CD8 beta. All three hybridomas released interleukin 2 upon incubation with L cells expressing Kd-peptide derivative complexes, though CD8 alpha/beta cells did so more efficiently than CD8 alpha/alpha and especially CD8-/- cells. More strikingly, only CD8 alpha/beta cells were able to recognize a weak agonist peptide derivative variant. This recognition was abolished by Fab' fragments of the anti-Kd alpha 3 monoclonal antibody SF1-1.1.1 or substitution of Kd D-227 with K, both conditions known to impair CD8 coreceptor function. T cell receptor (TCR) photoaffinity labeling indicated that TCR-ligand binding on CD8 alpha/beta cells was approximately 5- and 20-fold more avid than on CD8 alpha/a and CD8-/- cells, respectively. SF1-1.1.1 Fab' or Kd mutation D227K reduced the TCR photoaffinity labeling on CD8 alpha/beta cells to approximately the same low levels observed on CD8-/- cells. These results indicate that CD8 alpha/beta is a more efficient coreceptor than CD8alpha/alpha, because it more avidly strengthens TCR-ligand binding.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-1372996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-1396954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-1506684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-1534146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-1534242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-1547508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-2107545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-2109837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-2114552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-2128468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-2955903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-3116077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-3258885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-3262426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-6607120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7539035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7621078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7699318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7821755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7830771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7889415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-7889416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-8099195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-8108731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-8119892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-8478607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8976201-8533896
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2439-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8976201-Amino Acid Sequence,
pubmed-meshheading:8976201-Animals,
pubmed-meshheading:8976201-Antigens, CD8,
pubmed-meshheading:8976201-Cell Line,
pubmed-meshheading:8976201-Flow Cytometry,
pubmed-meshheading:8976201-H-2 Antigens,
pubmed-meshheading:8976201-Hybridomas,
pubmed-meshheading:8976201-Mice,
pubmed-meshheading:8976201-Peptide Fragments,
pubmed-meshheading:8976201-Plasmodium berghei,
pubmed-meshheading:8976201-Protozoan Proteins,
pubmed-meshheading:8976201-T-Lymphocytes,
pubmed-meshheading:8976201-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8976201-Thymoma,
pubmed-meshheading:8976201-Thymus Neoplasms
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pubmed:year |
1996
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pubmed:articleTitle |
CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding.
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pubmed:affiliation |
Centre d'Immunologie Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique de Marseille-Luminy, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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