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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-1-22
|
| pubmed:abstractText |
Chimeric receptors that redirect effector cell function to tumor cells or virus-infected cells have received much attention. Given the high affinity of Fc(epsilon)RI for immunoglobulin E (IgE) and low serum IgE levels, redirection of effector cells using Fc(epsilon) receptor may provide a novel, versatile, and effective anti-tumor strategy. We have used a mouse perforin 5'-promoter to express a single-chain human Fc(epsilon) receptor in the mouse cytotoxic T lymphocyte cell line, CTLL-R8. Upon ligation of the chimeric Fc(epsilon) receptors by IgE, a signal for effector function is transmitted via the intracellular domain of CD3zeta. Selection in G418-containing medium produced CTLLR8 transfectant clones that: (1) expressed chimeric Fc(epsilon) receptor as determined by flow cytometry; (2) bound human IgE antibodies with high affinity as determined by Scatchard analysis; (3) specifically rosetted IgE-coated SRBC; (4) lysed target cells in IgE-mediated ADCC and reverse ADCC assays; and (5) retarded tumor growth in a Winn assay. Therefore these chimeric Fc(epsilon) receptors can effectively redirect cytotoxicity to tumor cells. Future efforts will assess the versatility and efficacy of these IgE-binding chimeric receptors to redirect killer cell function in animal tumor models.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Perforin,
http://linkedlifedata.com/resource/pubmed/chemical/Pore Forming Cytotoxic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0741-5400
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
60
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
721-8
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8975874-Animals,
pubmed-meshheading:8975874-Antibody-Dependent Cell Cytotoxicity,
pubmed-meshheading:8975874-Antigens, CD3,
pubmed-meshheading:8975874-Cytotoxicity, Immunologic,
pubmed-meshheading:8975874-Dose-Response Relationship, Immunologic,
pubmed-meshheading:8975874-Humans,
pubmed-meshheading:8975874-Immunoglobulin E,
pubmed-meshheading:8975874-Immunotherapy,
pubmed-meshheading:8975874-Membrane Glycoproteins,
pubmed-meshheading:8975874-Mice,
pubmed-meshheading:8975874-Mice, Inbred C57BL,
pubmed-meshheading:8975874-Neoplasms, Experimental,
pubmed-meshheading:8975874-Perforin,
pubmed-meshheading:8975874-Pore Forming Cytotoxic Proteins,
pubmed-meshheading:8975874-Receptors, IgE,
pubmed-meshheading:8975874-Recombinant Fusion Proteins,
pubmed-meshheading:8975874-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8975874-Transfection
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
The use of chimeric human Fc(epsilon) receptor I to redirect cytotoxic T lymphocytes to tumors.
|
| pubmed:affiliation |
Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|