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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1996-12-30
|
| pubmed:abstractText |
Dissociation of an activated alpha-subunit from the beta-gamma complex directly regulates secondary messenger proteins. To address the potential role of G proteins expressed in human fetal cochlea, degenerate oligonucleotide primers corresponding to the 3'-end of the conserved region of alpha-subunits were used for polymerase chain reaction amplification of reverse-transcribed total human fetal cochlear mRNAs; GNAZ and GNAQ were isolated. These two G proteins are unique among the G-protein family because they lack a typical pertussis modification site. GNAZ is expressed in high levels in neural tissue while GNAQ is ubiquitously expressed. We characterized GNAZ expression using Northern blots, tissue in-situ hybridization and immunohistochemistry techniques to elucidate the potential role of this protein in inner ear function. Our data suggest that GNAZ may play a role in maintaining the ionic balance of perilymphatic and endolymphatic cochlear fluids.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0378-5955
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
90
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
55-64
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8975005-Autoradiography,
pubmed-meshheading:8975005-Base Sequence,
pubmed-meshheading:8975005-Blotting, Northern,
pubmed-meshheading:8975005-Cloning, Molecular,
pubmed-meshheading:8975005-Cochlea,
pubmed-meshheading:8975005-DNA,
pubmed-meshheading:8975005-Ear, Inner,
pubmed-meshheading:8975005-Fetal Proteins,
pubmed-meshheading:8975005-GTP-Binding Proteins,
pubmed-meshheading:8975005-Humans,
pubmed-meshheading:8975005-Immunohistochemistry,
pubmed-meshheading:8975005-In Situ Hybridization,
pubmed-meshheading:8975005-Molecular Sequence Data,
pubmed-meshheading:8975005-Perilymph,
pubmed-meshheading:8975005-Polymerase Chain Reaction,
pubmed-meshheading:8975005-RNA, Messenger,
pubmed-meshheading:8975005-Second Messenger Systems,
pubmed-meshheading:8975005-Transcription, Genetic
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
GNAZ in human fetal cochlea: expression, localization, and potential role in inner ear function.
|
| pubmed:affiliation |
Department of Genetics, Harvard Medical School, Boston, MA, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|