Linked Life Data

8974994

Source:http://linkedlifedata.com/resource/pubmed/id/8974994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-12-30
pubmed:abstractText
In a previous paper (Thalmann et al., 1993) we reported that the amino acid sequence of OCP2, a low molecular weight acidic protein present in extremely high concentrations in the organ of Corti and absent in the basilar papilla, exhibits a rudimentary EF-hand--a potential calcium-binding domain. The present study was undertaken to determine whether OCP2 binds 45-calcium under non-denaturing conditions following separation by isoelectric focusing and transblotting. The same criterion was used to determine whether the EF-hands of several other calcium-binding proteins (CBP) are functional in organ of Corti and basilar papilla. OCP2 exhibited no 45-calcium binding. Calmodulin, present in the organ of Corti in extremely high concentrations and lower in basilar papilla, showed strong 45-calcium binding in both structures. While calbindin represents a major protein in basilar papilla and binds 45-calcium, this protein is a minor component in the organ of Corti; whether it binds 45-calcium remains to be decided. By extending the pI range in the acidic region of isoelectric focusing, a 15 kDa, highly acidic (pI approximately 3.1) protein was revealed that constitutes a major protein in the organ of Corti; the protein was not detectable in the basilar papilla, spiral ligament/stria vascularis complex and numerous other organs tested. It remains to be resolved whether this protein represents an isoform of parvalbumin or a novel CBP. The differential make-up of CBPs between the mammalian organ of Corti and the avian basilar papilla is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0378-5955
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-75
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Calcium-binding proteins in organ of Corti and basilar papilla: CBP-15, an unidentified calcium-binding protein of the inner ear.
pubmed:affiliation
Department of Otolaryngology, Washington University, St. Louis MO 63110, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.