Linked Life Data

8971541

Source:http://linkedlifedata.com/resource/pubmed/id/8971541

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-3-20
pubmed:databankReference
pubmed:abstractText
The polymorphism of the human leucocyte antigen HLA-DR2 and the heterogeneity of HLA-DR2 class II-related haplotypes (HLA-DRB1-DRB5-DQA1-DQB1) were investigated in four populations of east and south-east Asia (SEA) and five Melanesian populations using TaqI restriction fragment length polymorphism (RFLP) analysis, and the polymerase chain reaction (PCR) amplification-based techniques PCR-RFLP and sequence-specific oligonucleotide (SSO) typing. The haplotype DRB1*1502-DRB5*0101-DQA1*0102-DQB1*0601 was common in Malaysians, Javanese, Thursday Islanders, Madang, Goroka and the Australian Aborigines, while DRB1*16021-DRB5*0101-DQA1*0102-DQB1*0502 was common in the Thai and Thursday Islanders. DRB1*1501-DRB5*0101-DQA1*0102-DQB1*0602 was present at a high frequency in Northern Chinese, Goroka, Watut and Australian Aborigines. The study describes four rare or unusual haplotypes: HLA-DRB1*1501-DRB5*0101-DQA1*0101-DQB1*0601, DRB1*1502-DRB5*0101-DQA1*0101-DQB1*0502, DRB1*1502-DRB5*0102-DQA1* 0102-DQB1*0502 and DRB1*1501-DRB5*0101-DQA1*0101/2-DQB1*0503; the latter two were confirmed by segregation in two Javanese families. A new DR2 allele, initially detected by PCR-RFLP and confirmed by DNA sequencing as DRB1*16022 (previously designated DRB1*16Madang), was seen in a Madang individual. A new HLA-DR2 TaqI RFLP subtype, locally designated as DR15U, is also described. This RFLP subtype segregated in a Javanese family and correlated with a typically SEA haplotype, DRB1*1502-DRB5*0102-DQA1*0101-DQB1*0501. The allele HLA-DR16Thai, determined by TaqI DRB RFLP, was found by PCR-RFLP and SSO typing to correlate with a unique SEA haplotype, HLA-DRB1*16021-DRB5*0101-DQA1*0102-DQB1*0502, and was observed in the Thai, Malaysian, Thursday Islander, Javanese and Northern Chinese populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0960-7420
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
437-49
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8971541-Alleles, pubmed-meshheading:8971541-Asia, Southeastern, pubmed-meshheading:8971541-Australia, pubmed-meshheading:8971541-Base Sequence, pubmed-meshheading:8971541-China, pubmed-meshheading:8971541-DNA, pubmed-meshheading:8971541-Genetic Variation, pubmed-meshheading:8971541-HLA-DQ Antigens, pubmed-meshheading:8971541-HLA-DQ alpha-Chains, pubmed-meshheading:8971541-HLA-DQ beta-Chains, pubmed-meshheading:8971541-HLA-DR Antigens, pubmed-meshheading:8971541-HLA-DR2 Antigen, pubmed-meshheading:8971541-HLA-DRB1 Chains, pubmed-meshheading:8971541-HLA-DRB5 Chains, pubmed-meshheading:8971541-Haplotypes, pubmed-meshheading:8971541-Humans, pubmed-meshheading:8971541-Molecular Sequence Data, pubmed-meshheading:8971541-Polymerase Chain Reaction, pubmed-meshheading:8971541-Polymorphism, Restriction Fragment Length, pubmed-meshheading:8971541-Population
pubmed:year
1996
pubmed:articleTitle
HLA-DR2 haplotypic diversity in populations of South-East Asia, northern China, Melanesia and Australian aborigines using PCR-RFLP for DRB1, DRB5, DQA1 and DQB1. A novel DRB1 allele: DRB1*16022.
pubmed:affiliation
Molecular Genetics, Tissue Typing, Red Cross Blood Transfusion Service, Sydney, New South Wales, Australia.
pubmed:publicationType
Journal Article