Linked Life Data

8970890

Source:http://linkedlifedata.com/resource/pubmed/id/8970890

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1997-4-3
pubmed:abstractText
The inositol trisphosphate receptor (IP3R) is an intracellular calcium channel that mediates the cellular actions of a wide variety of hormones, growth factors, and cytokines. In osteoblastic cell cultures, many bone resorbing hormones increase phosphoinositide turnover, inositol trisphosphate production, mobilization of intracellular calcium, and the secretion of osteoclast recruitment and activating factors. In this study, the effects of 17 beta-estradiol, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), phrobol ester, and serum on IP3R mRNA levels were evaluated in osteogenic-osteosarcoma cells and in primary osteoblastic cultures derived from neonatal rat calvaria. Type-specific reverse transcription polymerase chain reaction (RT-PCR) indicated that all cell types evaluated (G-292, U-2 OS, Saos-2, MC3T3-E1, UMR-106, and calvarial osteoblastic cells) express IP3R mRNA type I; G-292, U-2 OS, MC3T3-E1, and calvarial osteoblastic cells also express type II IP3R mRNA; and UMR-106 and the calvarial osteoblastic cells express type III IP3R mRNA. Northern blot and RT-PCR analyses of human G-292 osteosarcoma cells and rat calvarial osteoblastic cells showed that phorbol ester and serum increase IP3R mRNA levels, whereas 17 beta-estradiol and 1,25(OH)2D3 decrease these levels. In G-292 cells, the effect of 17 beta-estradiol was not due to accelerated IP3R mRNA degradation and required continued protein synthesis. The results show that multiple IP3R types are expressed in osteoblasts and osteoblastic osteosarcoma cells and that this expression is regulated by 17 beta-estradiol and other osteoporotic and antiosteoporotic hormones. These findings indicate that hormonal control of IP3R expression may be relevant in the chronic regulation of osteoblast secretory activity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0884-0431
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1889-96
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8970890-Animals, pubmed-meshheading:8970890-Blood Physiological Phenomena, pubmed-meshheading:8970890-Calcitriol, pubmed-meshheading:8970890-Calcium Channels, pubmed-meshheading:8970890-Cell Line, pubmed-meshheading:8970890-Down-Regulation, pubmed-meshheading:8970890-Estradiol, pubmed-meshheading:8970890-Gene Expression, pubmed-meshheading:8970890-Humans, pubmed-meshheading:8970890-Inositol 1,4,5-Trisphosphate, pubmed-meshheading:8970890-Inositol 1,4,5-Trisphosphate Receptors, pubmed-meshheading:8970890-Mice, pubmed-meshheading:8970890-Osteoblasts, pubmed-meshheading:8970890-Polymerase Chain Reaction, pubmed-meshheading:8970890-Rats, pubmed-meshheading:8970890-Rats, Sprague-Dawley, pubmed-meshheading:8970890-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:8970890-Tetradecanoylphorbol Acetate, pubmed-meshheading:8970890-Tumor Cells, Cultured, pubmed-meshheading:8970890-Up-Regulation
pubmed:year
1996
pubmed:articleTitle
Inositol trisphosphate receptor gene expression and hormonal regulation in osteoblast-like cell lines and primary osteoblastic cell cultures.
pubmed:affiliation
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.