Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
|
pubmed:dateCreated |
1997-1-17
|
pubmed:abstractText |
The performance characteristics of two bone alkaline phosphatase (ALP; EC 3.1.3.1) assays, a wheat germ agglutinin (WGA) precipitation assay and a new immunoadsorption assay (IAA), were compared. The within- and between-run imprecision of the IAA (3.6-4.2% and 3.6-7.7%) was comparable with that of the WGA assay. The mean cross-reactivity with liver ALP appeared to be 4% in the WGA assay and 11% in the IAA. The reference ranges in a group of 155 healthy Caucasian (pre)pubertal schoolgirls were: 149-401 U/L (total ALP, 30 degrees C), 105-349 U/L (bone ALP, 30 degrees C, WGA assay), and 58-205 U/L (bone ALP, 25 degrees C, IAA). Comparison of the WGA assay (x) with the IAA (y) demonstrated a correlation coefficient of 0.95 [Deming regression equation: y = (0.56 +/- 0.01)x + (2.0 +/- 1.5); Sy[symbol: see text]x = 5.3 U/L]. Correlation studies of the WGA assay and the IAA results with total ALP demonstrated r = 0.98 and 0.96, respectively.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0009-9147
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
42
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1970-4
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:8969634-Alkaline Phosphatase,
pubmed-meshheading:8969634-Bone and Bones,
pubmed-meshheading:8969634-Chemical Precipitation,
pubmed-meshheading:8969634-Child,
pubmed-meshheading:8969634-Female,
pubmed-meshheading:8969634-Humans,
pubmed-meshheading:8969634-Immunosorbent Techniques,
pubmed-meshheading:8969634-Isoenzymes,
pubmed-meshheading:8969634-Liver,
pubmed-meshheading:8969634-Reference Values,
pubmed-meshheading:8969634-Regression Analysis,
pubmed-meshheading:8969634-Sensitivity and Specificity,
pubmed-meshheading:8969634-Wheat Germ Agglutinins
|
pubmed:year |
1996
|
pubmed:articleTitle |
Immunoadsorption assay for bone alkaline phosphatase compared with wheat germ agglutinin precipitation assay in serum from (pre)pubertal girls.
|
pubmed:affiliation |
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands. klinlab@azvu.nl
|
pubmed:publicationType |
Journal Article,
Comparative Study
|