Linked Life Data

8969469

Source:http://linkedlifedata.com/resource/pubmed/id/8969469

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-3-11
pubmed:databankReference
pubmed:abstractText
The structural integrity of the Adh gene in several isogenic lines of Drosophila melanogaster was tested by Southern blot analysis using a 4.75 kilobase (kb) genomic clone of Alcohol dehydrogenase (Adh) as a probe. One line, RI22, III, showed evidence of a spontaneous insertion mutation 5' to the adult enhancer in an area previously indicated as a putative larval enhancer region. The inserted allele was present at an approximate frequency of 50% in relation to the uninserted wildtype allele. Isogenic lines were constructed of both homozygous mutant and wildtype flies, allowing the comparison of a spontaneous insertion mutant allele and it's direct wildtype ancestral allele. The inserted sequence is a 296 basepair (bp) truncated jockey retroposable element. The sequence and distribution of the element as well as it's proximity to the Adh gene are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0965-1748
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
593-8
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Molecular analysis of a spontaneous insertion mutation near the alcohol dehydrogenase gene of Drosophila melanogaster.
pubmed:affiliation
Department of Biology, University of Houston, Texas 77204-5513, USA.
pubmed:publicationType
Journal Article