Linked Life Data

8961935

Source:http://linkedlifedata.com/resource/pubmed/id/8961935

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
49
pubmed:dateCreated
1997-1-16
pubmed:databankReference
pubmed:abstractText
Infection of cells with picornaviruses of the rhino-, aphtho-, and enterovirus groups causes a shut-off in cap-dependent translation of cellular mRNAs but permits cap-independent viral RNA translation to proceed. This shut-off is thought to be mediated in part by the proteolytic cleavage of eukaryotic initiation factor 4G (eIF4G), although there is evidence to the contrary. Cleavage of eIF4G results in the separation of the eIF4E-binding domain from the ribosome- and helicase-binding domains of the factor, thereby limiting the ability of eIF4G to function in cap-dependent recruitment of mRNAs. Previously we determined the cleavage site within eIF4G targeted by the 2A proteases from human coxsackievirus serotype B4 and human rhinovirus serotype 2 using highly purified eIF4F and recombinant proteases. To examine further the role proteolysis of eIF4G plays in shut-off of translation, we altered the 2A cleavage site in human eIF4G by site-directed mutagenesis. Strikingly, the replacement of one amino acid at the 2A cleavage site resulted in a protein that is approximately 100-fold resistant to cleavage by coxsackievirus 2A protease and 10-50-fold for rhinovirus 2A. Alteration of the cleavage site had no effect on factor activity since the variant was just as active as wild-type eIF4G in restoring cap-dependent translation to an in vitro translation system depleted of endogenous eIF4G. Furthermore, the presence of the variant form of eIF4G rendered in vitro translation reactions resistant to the 2A protease-mediated inhibition of cap-dependent translation initiation. These results support the model that 2A proteases inhibit cap-dependent translation through direct proteolysis of eIF4G.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15726-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8961935-Animals, pubmed-meshheading:8961935-Blotting, Western, pubmed-meshheading:8961935-Cysteine Endopeptidases, pubmed-meshheading:8961935-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8961935-Endopeptidases, pubmed-meshheading:8961935-Enterovirus, pubmed-meshheading:8961935-Globins, pubmed-meshheading:8961935-Humans, pubmed-meshheading:8961935-Mutagenesis, Site-Directed, pubmed-meshheading:8961935-Oligodeoxyribonucleotides, pubmed-meshheading:8961935-Peptide Initiation Factors, pubmed-meshheading:8961935-Picornaviridae, pubmed-meshheading:8961935-Protein Biosynthesis, pubmed-meshheading:8961935-RNA, Messenger, pubmed-meshheading:8961935-RNA Cap-Binding Proteins, pubmed-meshheading:8961935-RNA-Binding Proteins, pubmed-meshheading:8961935-Rabbits, pubmed-meshheading:8961935-Recombinant Proteins, pubmed-meshheading:8961935-Reticulocytes, pubmed-meshheading:8961935-Rhinovirus, pubmed-meshheading:8961935-Viral Proteins
pubmed:year
1996
pubmed:articleTitle
A single amino acid change in protein synthesis initiation factor 4G renders cap-dependent translation resistant to picornaviral 2A proteases.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.