Linked Life Data

8961261

Source:http://linkedlifedata.com/resource/pubmed/id/8961261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1997-3-20
pubmed:abstractText
Regulation of gene expression by GH has so far been shown to be mediated by a few cis-acting elements, most of which are signal transducer and activator of transcription (STAT)-binding sites. Here we have characterized a novel GH-response element present in the promoter of rat serine protease inhibitor (spi) genes. It consists of a 13 nucleotide-long GAGA box containing two GAGGAG repeats separated by a G, structurally unrelated to STAT-binding sites. In hepatocytes, the spi GAGA box behaves as a position-dependent bifunctional enhancer controlling basal and GH-dependent transcription. In addition, spi GAGA box oligonucleotides inhibit cell-free transcription driven by GAGA box-containing as well as GAGA box-less promoters, suggesting that the spi GAGA box interacts directly or indirectly with component(s) of the basic transcriptional machinery. Mobility shift assays showed that this GAGA box is specifically recognized by nuclear factors that are unrelated to previously characterized proteins binding to purine-rich elements or to GH-activated STATs. Finally, experiments performed with cells expressing wild type, truncated, or mutated forms of the GH receptor indicate that protein kinase Janus kinase 2 is involved in the GH-dependent activation of the spi GAGA box. These studies reveal the existence of an as yet unidentified Janus kinase-2-dependent, STAT-independent pathway in GH activation of gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1507-18
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8961261-Animals, pubmed-meshheading:8961261-Binding Sites, pubmed-meshheading:8961261-CHO Cells, pubmed-meshheading:8961261-Cells, Cultured, pubmed-meshheading:8961261-Cricetinae, pubmed-meshheading:8961261-DNA-Binding Proteins, pubmed-meshheading:8961261-Enhancer Elements, Genetic, pubmed-meshheading:8961261-Gene Expression Regulation, pubmed-meshheading:8961261-Growth Hormone, pubmed-meshheading:8961261-Janus Kinase 2, pubmed-meshheading:8961261-Liver, pubmed-meshheading:8961261-Models, Biological, pubmed-meshheading:8961261-Nuclear Proteins, pubmed-meshheading:8961261-Promoter Regions, Genetic, pubmed-meshheading:8961261-Protein-Tyrosine Kinases, pubmed-meshheading:8961261-Proto-Oncogene Proteins, pubmed-meshheading:8961261-Rats, pubmed-meshheading:8961261-Recombinant Proteins, pubmed-meshheading:8961261-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:8961261-Serine Proteinase Inhibitors, pubmed-meshheading:8961261-Trans-Activators, pubmed-meshheading:8961261-Transcription, Genetic
pubmed:year
1996
pubmed:articleTitle
A novel growth hormone response element unrelated to STAT (signal transducer and activator of transcription)-binding sites is a bifunctional enhancer.
pubmed:affiliation
INSERM U-376, Hôpital Amaud de Villeneuve, Montpellier, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't