Linked Life Data

8959512

Source:http://linkedlifedata.com/resource/pubmed/id/8959512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-4-10
pubmed:abstractText
We constructed a phylogenetic tree based on the nucleotide sequence of the ure B gene of Helicobacter pylori strains, amplified by polymerase chain reaction (PCR). We analyzed the tree in terms of the relationship between the PCR-restriction fragment length polymorphism (RFLP)-genotype and the sequevar. The main frame of ure B (928 bp) was amplified by PCR from 38 strains of clinical isolates and reference species. The amplified probes were sequenced and processed into matrix and phylogenic trees. Homogeneity of the strains of "intra-case" origin and heterogeneity in "inter-cases" was parallel to clustering on the phylogenic tree. Sequevars in a cluster showed an identical RFLP-genotype in one case, indicating the clonal origin of H. pylori flora in an individual.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0944-1174
pubmed:author
pubmed:issnType
Print
pubmed:volume
31 Suppl 9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-23
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Strain differentiation and phylogenic relationships, in terms of base sequence of the ure B gene, of Helicobacter pylori.
pubmed:affiliation
Department of Bacteriology, Hyogo College of Medicine, Japan.
pubmed:publicationType
Journal Article