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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-2-25
|
| pubmed:abstractText |
Sixteen matching sera and DNA samples from healthy African blood donors living in rural areas of Guinea were analysed for the presence of type D retrovirus markers. Screening for the antibodies against structural proteins of Mason-Pfizer monkey virus (M-PMV) was carried out by Western blot with a purified M-PMV as an antigen. Eight out of 16 sera samples were found to contain antibodies against at least two gag gene-coded proteins, and three of these were weakly positive against env gene-coded protein. Using PCR amplification and Southern hybridization, we detected M-PMV-like gag sequences in 11 out of 16 samples and env-related sequences in 8 out of 16 samples. Six DNAs were found to contain both M-PMV gag- and env-related sequences. Restriction endonuclease analysis of the PCR-amplified gag sequences from two individuals and direct DNA sequencing analysis of the amplimers confirmed their M-PMV-like origin. Detection of antibodies and M-PMV-related sequences in blood donors from Guinea, but not in French or Algerian blood donors, indicated exogenous SRV infection in humans from certain geographic areas of Western Africa.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Structural Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0923-2516
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
147
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
341-51
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8958587-Adolescent,
pubmed-meshheading:8958587-Adult,
pubmed-meshheading:8958587-Antibodies, Viral,
pubmed-meshheading:8958587-Biological Markers,
pubmed-meshheading:8958587-Blotting, Southern,
pubmed-meshheading:8958587-Blotting, Western,
pubmed-meshheading:8958587-Cell Line,
pubmed-meshheading:8958587-Child,
pubmed-meshheading:8958587-DNA, Viral,
pubmed-meshheading:8958587-DNA Restriction Enzymes,
pubmed-meshheading:8958587-Female,
pubmed-meshheading:8958587-Guinea,
pubmed-meshheading:8958587-Humans,
pubmed-meshheading:8958587-Male,
pubmed-meshheading:8958587-Mason-Pfizer monkey virus,
pubmed-meshheading:8958587-Pilot Projects,
pubmed-meshheading:8958587-Polymerase Chain Reaction,
pubmed-meshheading:8958587-Retroviridae Infections,
pubmed-meshheading:8958587-Tumor Virus Infections,
pubmed-meshheading:8958587-Viral Structural Proteins
|
| pubmed:articleTitle |
Type D retrovirus markers in healthy Africans from Guinea.
|
| pubmed:affiliation |
Laboratory of Molecular Virology and Carcinogenesis, NCI-Frederick Cancer Research and Development Center, MD 21702, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|