Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-1-7
|
| pubmed:abstractText |
To evaluate whether the detection of Toxoplasma gondii DNA in CSF could contribute to the differential diagnosis of AIDS-related focal brain lesions, CSF samples from 88 HIV-infected patients (56 with focal brain lesions and 32 without) were tested prospectively by a nested PCR for the B1 gene of T. gondii. The assay had a detection limit of 10 trophozoite equivalents. Six of 18 patients with toxoplasmic encephalitis, but none of the 70 patients with other disorders, were PCR-positive (33.3% sensitivity and 100% specificity). Considering only those patients with cerebral toxoplasmosis from whom CSF was collected before or during the first week of antitoxoplasmic therapy, sensitivity rose to 50%. This was higher than the sensitivity in patients whose CSF was collected after the first week of treatment (odds ratio (OR) of 7.0; 95% CI: 0.46-218.2). The administration of antitoxoplasmic prophylaxis did not affect the PCR results. Patients with a poor response to therapy had a higher probability of detectable T. gondii DNA in their CSF (OR of 5.0; 95% CI: 0.37-86.6). All patients with other central nervous system disorders were PCR-negative. Despite the moderate sensitivity, the high specificity and positive predictive value (100%) make this assay a useful tool in the differential diagnosis of AIDS-related focal brain lesions as part of a series of CSF and neuroradiological examinations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-2615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
45
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
472-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8958252-AIDS-Related Opportunistic Infections,
pubmed-meshheading:8958252-Adult,
pubmed-meshheading:8958252-Animals,
pubmed-meshheading:8958252-Antibodies, Protozoan,
pubmed-meshheading:8958252-Coccidiostats,
pubmed-meshheading:8958252-Cohort Studies,
pubmed-meshheading:8958252-Confidence Intervals,
pubmed-meshheading:8958252-DNA, Protozoan,
pubmed-meshheading:8958252-Diagnosis, Differential,
pubmed-meshheading:8958252-Female,
pubmed-meshheading:8958252-Humans,
pubmed-meshheading:8958252-Immunoglobulin G,
pubmed-meshheading:8958252-Male,
pubmed-meshheading:8958252-Middle Aged,
pubmed-meshheading:8958252-Odds Ratio,
pubmed-meshheading:8958252-Polymerase Chain Reaction,
pubmed-meshheading:8958252-Prospective Studies,
pubmed-meshheading:8958252-Sensitivity and Specificity,
pubmed-meshheading:8958252-Spinal Puncture,
pubmed-meshheading:8958252-Toxoplasma,
pubmed-meshheading:8958252-Toxoplasmosis, Cerebral
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
PCR detection of Toxoplasma gondii DNA in CSF for the differential diagnosis of AIDS-related focal brain lesions.
|
| pubmed:affiliation |
Department of Infectious Diseases, Catholic University, Rome, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|