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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
51
|
| pubmed:dateCreated |
1997-1-23
|
| pubmed:databankReference | |
| pubmed:abstractText |
Although coordinate expression of carbohydrate epitopes during development is well described, mechanisms which regulate this expression remain largely unknown. In this study we demonstrate that developing chicken B cells express the LewisX terminal oligosaccharide structure in a stage-specific manner. To examine regulation of this expression, we have cloned and expressed the chicken alpha(1,3)-fucosyltransferase gene involved in LewisX biosynthesis, naming it chicken fucosyltransferase 1 (CFT1). CFT1 is characterized by a single long open reading frame of 356 amino acids encoding a type II transmembrane glycoprotein. The domain structure and predicted amino acid sequence are highly conserved between CFT1 and mammalian FucTIV genes (52.8% and 46.3% identity to mouse and human respectively). In vitro CFT1 fucosyltransferase activity utilizes LacNAc > 3'sialyl-LacNAc acceptors with almost no utilization of other neutral type II (lactose, 2-fucosyllactose), or type I (lacto-N-biose I) acceptors. CFT1-transfected cells make cell surface LewisX (COS-7) and LewisX + VIM-2 structures (Chinese hamster ovary). CFT1 gene expression is tissue-specific and includes embryonic thymus and bursa. Furthermore, expression of the CFT1 gene and cell surface LewisX structures are closely linked during B cell development. These findings reveal the evolutionary conservation between nonmammalian and mammalian alpha(1,3)-fucosyltransferase genes and demonstrate a role for fucosyltransferase gene regulation in the developmental expression of oligosaccharide structures.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD15,
http://linkedlifedata.com/resource/pubmed/chemical/Fucosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/galactoside 3-fucosyltransferase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
271
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
32960-7
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| pubmed:dateRevised |
2005-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8955139-Amino Acid Sequence,
pubmed-meshheading:8955139-Animals,
pubmed-meshheading:8955139-Antigens, CD15,
pubmed-meshheading:8955139-B-Lymphocytes,
pubmed-meshheading:8955139-Base Sequence,
pubmed-meshheading:8955139-COS Cells,
pubmed-meshheading:8955139-Chickens,
pubmed-meshheading:8955139-Cloning, Molecular,
pubmed-meshheading:8955139-Fucosyltransferases,
pubmed-meshheading:8955139-Gene Expression Regulation, Developmental,
pubmed-meshheading:8955139-Genes,
pubmed-meshheading:8955139-Membrane Glycoproteins,
pubmed-meshheading:8955139-Molecular Sequence Data,
pubmed-meshheading:8955139-RNA, Messenger,
pubmed-meshheading:8955139-Restriction Mapping,
pubmed-meshheading:8955139-Sequence Alignment,
pubmed-meshheading:8955139-Sequence Homology, Amino Acid,
pubmed-meshheading:8955139-Tissue Distribution
|
| pubmed:year |
1996
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| pubmed:articleTitle |
Molecular cloning and characterization of CFT1, a developmentally regulated avian alpha(1,3)-fucosyltransferase gene.
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| pubmed:affiliation |
Immune Cell Biology Program, Naval Medical Research Institute, Bethesda, Maryland 20889, USA. rin0kxl@bumed30.med.navy.mil
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| pubmed:publicationType |
Journal Article
|