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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-1-7
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pubmed:abstractText |
To clarify the role of vitamin E (alpha-tocopherol) for the induction of cyclooxygenase-2 (COX-2) in rat macrophages stimulated by lipopolysaccharide (LPS), vitamin E-enriched macrophages were prepared by intraperitoneal injection of vitamin E for 6 days at a rate of 5 mg per day. The production of PGE2 was increased in dose- and time-dependent manners by addition of LPS in both control and vitamin E-enriched peritoneal macrophages. The maximum effect of LPS was observed in 12 h at concentration of 5 micrograms/ml. By analyzing COX-2 mRNA level by Northern blot and COX-2 enzyme mass and phosphotyrosine by Western blot, it was revealed that the increase of PGE2 production reflected the induction of COX-2 expression through activation of tyrosine kinase. Vitamin E failed to inhibit PGE2 production in LPS-stimulated macrophages; however, genistein, a tyrosine kinase inhibitor, completely inhibited the production at 100 microM. These results suggest that vitamin E does not inhibit COX-2 expression via LPS-mediated tyrosine kinase signal transduction pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp...,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radical Scavengers,
http://linkedlifedata.com/resource/pubmed/chemical/Genistein,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Isoflavones,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin E
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
1304
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-44
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8954137-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:8954137-Animals,
pubmed-meshheading:8954137-Antioxidants,
pubmed-meshheading:8954137-Cyclooxygenase 2,
pubmed-meshheading:8954137-Dinoprostone,
pubmed-meshheading:8954137-Dose-Response Relationship, Drug,
pubmed-meshheading:8954137-Enzyme Induction,
pubmed-meshheading:8954137-Enzyme Inhibitors,
pubmed-meshheading:8954137-Free Radical Scavengers,
pubmed-meshheading:8954137-Genistein,
pubmed-meshheading:8954137-Isoenzymes,
pubmed-meshheading:8954137-Isoflavones,
pubmed-meshheading:8954137-Lipopolysaccharides,
pubmed-meshheading:8954137-Macrophages, Peritoneal,
pubmed-meshheading:8954137-Male,
pubmed-meshheading:8954137-Phosphorylation,
pubmed-meshheading:8954137-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:8954137-Protein-Tyrosine Kinases,
pubmed-meshheading:8954137-Rats,
pubmed-meshheading:8954137-Rats, Wistar,
pubmed-meshheading:8954137-Signal Transduction,
pubmed-meshheading:8954137-Vitamin E
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pubmed:year |
1996
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pubmed:articleTitle |
Effect of vitamin E on expression of cyclooxygenase-2 in lipopolysaccharide-stimulated rat macrophages.
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pubmed:affiliation |
Department of Biochemistry, School of Dentistry, Sapporo, Japan.
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pubmed:publicationType |
Journal Article
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