Linked Life Data

8953714

Source:http://linkedlifedata.com/resource/pubmed/id/8953714

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1997-1-3
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007254, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58338, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58339, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58340, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58341, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58342, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58343, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58344, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58345, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58346, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58347, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58348, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58349, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58350, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58351, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58352, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58353, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58354, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58355, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U58356
pubmed:abstractText
Culturable bacteria that were numerically important members of a marine enrichment community were identified and characterized phylogenetically. Selective and nonselective isolation methods were used to obtain 133 culturable bacterial isolates from model marine communities enriched with the high-molecular-weight (lignin-rich) fraction of pulp mill effluent. The culture collection was screened against community DNA from the lignin enrichments by whole-genome hybridization methods, and three marine bacterial isolates were identified as being numerically important in the communities. One isolate was in the alpha-subclass of Proteobacteria, and the other two were in the gamma-subclass of Proteobacteria. Isolate-specific 16S rRNA oligonucleotide probes designed to precisely quantify the isolates in the lignin enrichment communities indicated contributions ranging from 2 to 32% of enrichment DNA, values nearly identical to those originally obtained by the simpler whole-genome hybridization method. Two 16S rRNA sequences closely related to that of one of the isolates, although not identical, were amplified via PCR from the seawater sample originally used to inoculate the enrichment medium. Partial sequences of 14 other isolates revealed significant phylogenetic diversity and unusual sequences among the culturable lignin enrichment bacteria, with the Proteobacteria, Cytophaga-Flavobacterium, and gram-positive groups represented.
pubmed:commentsCorrections
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pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4433-40
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Identifying numerically abundant culturable bacteria from complex communities: an example from a lignin enrichment culture.
pubmed:affiliation
Department of Microbiology, University of Georgia, Athens 30602, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't