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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1997-1-23
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pubmed:databankReference |
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pubmed:abstractText |
To examine the role of BMP signaling during limb pattern formation, we isolated chicken cDNAs encoding type I (BRK-1 and BRK-2) and type II (BRK-3) receptors for bone morphogenetic proteins. BRK-2 and BRK-3, which constitute dual-affinity signaling receptor complexes for BMPs, are co-expressed in condensing precartilaginous cells, while BRK-1 is weakly expressed in the limb mesenchyme. BRK-3 is also expressed in the apical ectodermal ridge and interdigital limb mesenchyme. BRK-2 is intensely expressed in the posterior-distal region of the limb bud. During digit duplication by implanting Sonic hedgehog-producing cells, BRK-2 expression is induced anteriorly in the new digit forming region as observed for BMP-2 and BMP-7 expression in the limb bud. Dominant-negative effects on BMP signaling were obtained by over-expressing kinase domain-deficient forms of the receptors. Chondrogenesis of limb mesenchymal cells is markedly inhibited by dominant-negative BRK-2 and BRK-3, but not by BRK-1. Although the bone pattern was not disturbed by expressing individual dominant-negative BRK independently, preferential distal and posterior limb truncations resulted from co-expressing the dominant-negative forms of BRK-2 and BRK-3 in the whole limb bud, thus providing evidence that BMPs are essential morphogenetic signals for limb bone patterning.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hedgehog Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-1991
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3557-66
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8951071-Amino Acid Sequence,
pubmed-meshheading:8951071-Animals,
pubmed-meshheading:8951071-Bone Morphogenetic Protein Receptors,
pubmed-meshheading:8951071-Bone Morphogenetic Protein Receptors, Type I,
pubmed-meshheading:8951071-Bone Morphogenetic Protein Receptors, Type II,
pubmed-meshheading:8951071-Bone Morphogenetic Proteins,
pubmed-meshheading:8951071-Bone and Bones,
pubmed-meshheading:8951071-Cartilage,
pubmed-meshheading:8951071-Cells, Cultured,
pubmed-meshheading:8951071-Chickens,
pubmed-meshheading:8951071-Extremities,
pubmed-meshheading:8951071-Genes, Dominant,
pubmed-meshheading:8951071-Hedgehog Proteins,
pubmed-meshheading:8951071-Molecular Sequence Data,
pubmed-meshheading:8951071-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8951071-Proteins,
pubmed-meshheading:8951071-Receptors, Cell Surface,
pubmed-meshheading:8951071-Receptors, Growth Factor,
pubmed-meshheading:8951071-Sequence Alignment,
pubmed-meshheading:8951071-Sequence Homology, Amino Acid,
pubmed-meshheading:8951071-Signal Transduction,
pubmed-meshheading:8951071-Trans-Activators
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pubmed:year |
1996
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pubmed:articleTitle |
BMP signaling during bone pattern determination in the developing limb.
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pubmed:affiliation |
Department of Molecular Biology, Kawasaki Medical School, Kurashiki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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