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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-1-16
|
| pubmed:databankReference | |
| pubmed:abstractText |
A 2432-bp cDNA for mouse FAD-linked glycerol-3-phosphate dehydrogenase, a nuclear-encoded enzyme associated with the inner mitochondrial membrane, was isolated from a Lambda ZAP phage library generated from brown adipocyte mRNA. The amino acid sequence was 95 and 93% homologous to the rat and human enzymes. PvuII and SacI polymorphisms between Mus spretus and C57BL/6J were used to map the mouse FAD-linked glycerol-3-phosphate dehydrogenase gene (Gdm1) to chromosome 2, 33 cM from the centromere. Northern blot analysis showed that brown adipose tissue predominantly expressed a 6.5-kb mRNA with lower expression of 4.5- and 2.4-kb forms, whereas brain and pancreatic islets almost exclusively expressed a 6.5-kb transcript, muscle expressed a 4.5-kb transcript, and testis expressed a 2.4-kb RNA form. Analysis of poly(A)+ RNA from brown adipose tissue suggested that all RNA forms are polyadenylated. Among the tissues examined, FAD-linked glycerol-3-phosphate dehydrogenase protein levels and enzyme activity were highest in brown adipose tissue, and a consistent correlation between protein levels and enzyme activity in all tissues was observed. RNA levels corresponded to protein levels in all tissues except testis, where high levels of 2.4-kb mRNA and relatively low protein were expressed. Exposing mice to cold temperatures induced the 6.5-kb mRNA and enzyme activity only in brown adipose tissue, suggesting a role for thermogenesis in this tissue. Although the molecular basis for the formation of the 6.5- and 4.5-kb mRNA's is not known, data suggest that the regulation of FAD-linked glycerol-3-phosphate dehydrogenase is complex and tissue-specific.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0003-9861
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
336
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
97-104
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8951039-Adipose Tissue, Brown,
pubmed-meshheading:8951039-Amino Acid Sequence,
pubmed-meshheading:8951039-Animals,
pubmed-meshheading:8951039-Base Sequence,
pubmed-meshheading:8951039-Binding Sites,
pubmed-meshheading:8951039-Chromosome Mapping,
pubmed-meshheading:8951039-Cloning, Molecular,
pubmed-meshheading:8951039-Cold Temperature,
pubmed-meshheading:8951039-Flavin-Adenine Dinucleotide,
pubmed-meshheading:8951039-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8951039-Glycerolphosphate Dehydrogenase,
pubmed-meshheading:8951039-Humans,
pubmed-meshheading:8951039-Mice,
pubmed-meshheading:8951039-Mice, Inbred C57BL,
pubmed-meshheading:8951039-Mice, Transgenic,
pubmed-meshheading:8951039-Mitochondria,
pubmed-meshheading:8951039-Molecular Sequence Data,
pubmed-meshheading:8951039-Rats,
pubmed-meshheading:8951039-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8951039-Tissue Distribution
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Sequence and tissue-dependent RNA expression of mouse FAD-linked glycerol-3-phosphate dehydrogenase.
|
| pubmed:affiliation |
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|