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pubmed-article:8948662pubmed:abstractTextWe investigated the influence of different buffers (Tris-buffer and phosphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin antibody (aPT) measurement by enzyme-linked immunosorbent assay (ELISA), employing a gamma-ray-irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris-buffer is not suitable for aPT measurement with a gamma-ray-irradiated plate to measure aPT.lld:pubmed
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pubmed-article:8948662pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8948662pubmed:articleTitleBuffer may be the critical factor in measurement of anti-prothrombin antibody on a gamma-ray-irradiated plate by enzyme-linked immunosorbent assay.lld:pubmed
pubmed-article:8948662pubmed:affiliationDepartment of Medicine, Teikyo University School of Medicine, Tokyo, Japan.lld:pubmed
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pubmed-article:8948662pubmed:publicationTypeComparative Studylld:pubmed