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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
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|
pubmed:dateCreated |
1997-1-13
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|
pubmed:abstractText |
We investigated the influence of different buffers (Tris-buffer and phosphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin antibody (aPT) measurement by enzyme-linked immunosorbent assay (ELISA), employing a gamma-ray-irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris-buffer is not suitable for aPT measurement with a gamma-ray-irradiated plate to measure aPT.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Dec
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|
pubmed:issn |
0361-8609
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
53
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|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
242-4
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|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
pubmed-meshheading:8948662-Autoantibodies,
pubmed-meshheading:8948662-Autoimmune Diseases,
pubmed-meshheading:8948662-Buffers,
pubmed-meshheading:8948662-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8948662-False Negative Reactions,
pubmed-meshheading:8948662-Gamma Rays,
pubmed-meshheading:8948662-Humans,
pubmed-meshheading:8948662-Lupus Erythematosus, Systemic,
pubmed-meshheading:8948662-Phosphates,
pubmed-meshheading:8948662-Polysorbates,
pubmed-meshheading:8948662-Prothrombin,
pubmed-meshheading:8948662-Reproducibility of Results,
pubmed-meshheading:8948662-Sodium Chloride,
pubmed-meshheading:8948662-Tromethamine
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|
pubmed:year |
1996
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|
pubmed:articleTitle |
Buffer may be the critical factor in measurement of anti-prothrombin antibody on a gamma-ray-irradiated plate by enzyme-linked immunosorbent assay.
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pubmed:affiliation |
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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