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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-1-6
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pubmed:abstractText |
The binding of tetrahydropteridines with 6-di- and trihydroxypropyl side chains to recombinant rat neuronal nitric oxide (NO) synthase (EC 1.14.13.39) was determined by competition with 6R-[3'-3H]-5,6,7,8-tetrahydro-L-erythro-biopterin (6R-[3'-3H]H4biopterin). Although all but one of the derivatives exhibited only poor affinities (Ki 50 microM), the 4-amino analogue of 6R-H4 biopterin was a potent antagonist of 6R-H4 biopterin binding (Ki 13.2 nM). The 4-amino analogue of 6R-H4 biopterin inhibited NO synthase stimulation by the natural cofactor 6R-H4 biopterin with an IC50 of 1 microM without affecting the basal activity observed in the absence of added 6R-H4 biopterin. Because the 4-amino analogue of 6R-H4biopterin also inhibited dihydropteridine reductase (EC 1.6.99.7; IC50 20 microM), our results support the hypothesis that redox cycling of H4 biopterin might be required for the NO synthase reaction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-1714584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-1715290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-1852778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-2015420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-4405600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7514595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7528005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7529491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7532811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7539994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7543842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7543976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-7691806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-8533013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-8626754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-8631754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8947486-8782608
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
320 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8947486-Animals,
pubmed-meshheading:8947486-Biopterin,
pubmed-meshheading:8947486-Brain,
pubmed-meshheading:8947486-Dihydropteridine Reductase,
pubmed-meshheading:8947486-Kinetics,
pubmed-meshheading:8947486-Neurons,
pubmed-meshheading:8947486-Nitric Oxide Synthase,
pubmed-meshheading:8947486-Rats,
pubmed-meshheading:8947486-Recombinant Proteins,
pubmed-meshheading:8947486-Sheep,
pubmed-meshheading:8947486-Spodoptera
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pubmed:year |
1996
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pubmed:articleTitle |
Identification of the 4-amino analogue of tetrahydrobiopterin as a dihydropteridine reductase inhibitor and a potent pteridine antagonist of rat neuronal nitric oxide synthase.
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pubmed:affiliation |
Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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