Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1997-1-8
pubmed:abstractText
Capillary isoelectric focusing was applied to determine the pI value of human rhinovirus serotype 2 (HRV 2), a picornavirus of about 8,500,000 Da in size. Using fused silica capillaries dynamically coated with hydroxypropylmethyl cellulose (added at 0.08% to the catholyte), the virus zone failed to reach the steady state position in the pH gradient within times usually employed in focusing experiments, as the electroosmotic flow (EOF) pushed the analyte zone past the detector. Therefore, the residence time of the zones in the separation capillary was extended by applying hydrodynamic pressure at the detector side during focusing, thus pneumatically counteracting the EOF. After completion of focusing, the zones were mobilized by pressure maintaining the high voltage. For calibration of the pH gradient, low molecular mass pI marker substances were employed. Using the relation between the apparent pI value of the virus and the focusing time under counter pressure, the actual pI of HRV2 was determined as 6.8 by extrapolating to infinite time.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4300-3
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Determination of the pI of human rhinovirus serotype 2 by capillary isoelectric focusing.
pubmed:affiliation
Institute of Analytical Chemistry, University of Vienna, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't