Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4 Pt 1
|
| pubmed:dateCreated |
1997-5-20
|
| pubmed:abstractText |
Cytolysis by NK cells that possess the NKB1 killer cell inhibitory receptor is inhibited by target cell expression of Bw4+ HLA-B molecules. The inhibitory effect can be prevented by addition of mAbs which block recognition of class I molecules by NKB1. The epitopes recognized by two anti-class I mAbs, DX15 and DX16, which inhibit the interaction of NKB1 with class I have been characterized. Binding of DX15 and DX16 to class I allotypes was investigated by flow cytometric analysis of transfected cell lines which express just one HLA-A, B, or C allele, and by immunoprecipitation of class I molecules from HLA typed B-lymphoblastoid cell lines, followed by isoelectric focusing. The DX16 mAb recognizes class I allotypes which possess alanine at position 71 of the alpha 1 helix, and therefore has a specificity resembling that of the ME1 mAb but with broader specificity. Class I recognition by DX15 is affected by polymorphisms of the C-terminal part of the alpha 1 helix, and the N-terminal part of the alpha 2 helix. DX15 thus appears to recognize a complex epitope near the end of the peptide binding groove which may be conformationally determined. Both antibodies are as effective as the anti-NKB1 mAb (DX9) in preventing class I recognition by the NKB1 receptor. DX16 also blocked recognition by a B*0702 allospecific CTL clone, whereas DX15 did not.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-C Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, KIR3DL1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0001-2815
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
48
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
278-84
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8946681-Alanine,
pubmed-meshheading:8946681-Animals,
pubmed-meshheading:8946681-Antibodies, Monoclonal,
pubmed-meshheading:8946681-Antibody Specificity,
pubmed-meshheading:8946681-Cell Line, Transformed,
pubmed-meshheading:8946681-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:8946681-Epitopes,
pubmed-meshheading:8946681-HLA-A Antigens,
pubmed-meshheading:8946681-HLA-B Antigens,
pubmed-meshheading:8946681-HLA-C Antigens,
pubmed-meshheading:8946681-Humans,
pubmed-meshheading:8946681-Killer Cells, Natural,
pubmed-meshheading:8946681-Mice,
pubmed-meshheading:8946681-Mice, Inbred BALB C,
pubmed-meshheading:8946681-Receptors, Immunologic,
pubmed-meshheading:8946681-Receptors, KIR,
pubmed-meshheading:8946681-Receptors, KIR3DL1,
pubmed-meshheading:8946681-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8946681-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Specificity of two anti-class I HLA monoclonal antibodies that block class I recognition by the NKB1 killer cell inhibitory receptor.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Stanford University, California, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|