Linked Life Data

8943857

Source:http://linkedlifedata.com/resource/pubmed/id/8943857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1996-12-31
pubmed:abstractText
CD1a+ dendritic cells (DC) differentiate from a major population of nonadherent CD13(hi)lin- cells that appear when human cord blood CD34+ hematopoietic progenitor cells are cultured with stem-cell factor, granulocyte/macrophage (MA) colony-stimulating factor, and tumor necrosis factor-alpha (TNF-alpha) for 5 days. CD13hilin- cells, which also comprise MA and granulocyte precursors, are CD4+ and can thus be targets of human immunodeficiency virus (HIV). Low replication was noted when these day 5 cells were infected with lymphotropic HIV-1LA1 (p24: < or = 4 ng/mL on day 8 postinfection [PI]), while high virus production occurred with MA-tropic HIV-1Ba-L, HIV-1Ada, or HIV-1-m-n. (p24: 50 to > or = 1,000 ng/mL). Strong cytopathicity (CPE) was then observed in nonadherent cells as in adherent MA. However, FACS analysis on day 7 PI showed that HIV did not affect differentiation of DC that survived CPE: apart from CD4 downmodulation related to HIV production, overall expression of CD40, CD80, and CD86 costimulatory molecules, and of HLA-DR, was unchanged relative to controls. At that time, the capacity of DC from HIV-infected cultures to stimulate the mixed leukocyte reaction was only altered less than 10-fold. Immunocytochemistry on day 7 PI showed that most HIV-infected cells were included in syncytia that were stained by anti-CD1a, anti-S100, and anti-CD14 antibodies, indicating that syncytia consisted of DC and cells of the MA lineage. Polymerase chain reaction analysis of FACS-sorted CD1a+ cells confirmed that they harbored then HIV DNA. Viral DNA was also detected in CD1a+ DC from noninfected cultures that had been exposed to HIV only after sorting. Therefore, we examined whether in infected cultures DC precursors were infected at the onset or if virus spread later from other infected cells to differentiated DC. This was answered by showing that, 24 hours postexposure to HIV, viral DNA was preferentially detected in day 5 sorted CD13hilin- versus CD13hilin- cells, and that it was found in the CD1a+ progeny of CD13(hi)lin- cells 48 hours later. In addition, HIV replication did not affect myeloid clonogenic progenitors in day 0 to day 7 PI cultures, although viral DNA was detected in colony-forming unit-granulocyte/macrophage (CFU-GM)/CFU-M colonies derived from day 3 and 7 PI cultures. Thus, precursors of DC and their progeny are susceptible to HIV in vitro, but, apart from CPE, the effect of virus production on DC differentiation or function is limited.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4215-28
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8943857-Antigens, CD, pubmed-meshheading:8943857-Antigens, CD1, pubmed-meshheading:8943857-Antigens, CD13, pubmed-meshheading:8943857-Antigens, CD34, pubmed-meshheading:8943857-Antigens, CD40, pubmed-meshheading:8943857-Antigens, CD80, pubmed-meshheading:8943857-Antigens, CD86, pubmed-meshheading:8943857-Cell Differentiation, pubmed-meshheading:8943857-Clone Cells, pubmed-meshheading:8943857-Colony-Forming Units Assay, pubmed-meshheading:8943857-Cytopathogenic Effect, Viral, pubmed-meshheading:8943857-DNA, Viral, pubmed-meshheading:8943857-Dendritic Cells, pubmed-meshheading:8943857-Fetal Blood, pubmed-meshheading:8943857-HIV Infections, pubmed-meshheading:8943857-HIV-1, pubmed-meshheading:8943857-Humans, pubmed-meshheading:8943857-Lymphocyte Culture Test, Mixed, pubmed-meshheading:8943857-Membrane Glycoproteins, pubmed-meshheading:8943857-Polymerase Chain Reaction, pubmed-meshheading:8943857-Proviruses, pubmed-meshheading:8943857-Virus Replication
pubmed:year
1996
pubmed:articleTitle
The effect of in vitro human immunodeficiency virus infection on dendritic-cell differentiation and function.
pubmed:affiliation
Laboratoire de Biologie et Pathologie des Déficits Immunitaires, l'Ecole Pratique des Hautes Etudes, Faculté de Médecine, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't