Linked Life Data

8943422

Source:http://linkedlifedata.com/resource/pubmed/id/8943422

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:8943422rdf:typepubmed:Citationlld:pubmed
pubmed-article:8943422lifeskim:mentionsumls-concept:C0035820lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0332307lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0034721lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0034693lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0017526lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0021760lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0079460lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0085236lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C1280500lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0017262lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C1333196lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C0443199lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C1159946lld:lifeskim
pubmed-article:8943422lifeskim:mentionsumls-concept:C1522492lld:lifeskim
pubmed-article:8943422pubmed:issue11lld:pubmed
pubmed-article:8943422pubmed:dateCreated1996-12-27lld:pubmed
pubmed-article:8943422pubmed:abstractTextMacrophage colony-stimulating factor (M-CSF) and granulocyte-macrophage (GM)-CSF stimulate the differentiation of rat alveolar macrophages (AM) into multinucleated giant cells (MGC) with distinct phenotypes (type 1 and type 2 MGC). In the present study, we analyzed the profile of cytokine gene expression induced respectively, by M-CSF and GM-CSF during rat AM differentiation using reverse transcription-PCR. Enhanced mRNA expression for IL-1alpha, TNF-alpha, and TGF-beta1 was observed 3 h after treatment with M-CSF (50 U/ml) or GM-CSF (50 U/ml). In contrast, IL-6 mRNA expression was increased by GM-CSF but not M-CSF. Kinetic analysis indicated that GM-CSF stimulated IL-6 expression early (1.5 h), with maximal effect observed at 24 h and up to 5 days thereafter. Increased mRNA levels for IL-6 were associated with higher IL-6 activity in the culture media of differentiating AM. IL-6 activity was stimulated 3 h after treatment with GM-CSF and increased with time (up to 5 days). Interestingly, addition of exogenous IL-6 (20-100 ng/ml) alone or in combination with GM-CSF to AM cultures increased slightly and selectively the formation of MGC with type 2 phenotype. Conversely, neutralization of endogenous IL-6 during AM differentiation into MGC inhibited significantly (up to 50%) the formation of type 2 MGC. These results suggest a role for IL-6 in the formation of type 2 MGC and provide some insights into the mechanisms of MGC formation and the processes that regulate it positively.lld:pubmed
pubmed-article:8943422pubmed:languageenglld:pubmed
pubmed-article:8943422pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:citationSubsetAIMlld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8943422pubmed:statusMEDLINElld:pubmed
pubmed-article:8943422pubmed:monthDeclld:pubmed
pubmed-article:8943422pubmed:issn0022-1767lld:pubmed
pubmed-article:8943422pubmed:authorpubmed-author:DornandJJlld:pubmed
pubmed-article:8943422pubmed:authorpubmed-author:YangHHlld:pubmed
pubmed-article:8943422pubmed:authorpubmed-author:LemaireIIlld:pubmed
pubmed-article:8943422pubmed:authorpubmed-author:CommesTTlld:pubmed
pubmed-article:8943422pubmed:authorpubmed-author:LafontVVlld:pubmed
pubmed-article:8943422pubmed:authorpubmed-author:CantinM FMFlld:pubmed
pubmed-article:8943422pubmed:issnTypePrintlld:pubmed
pubmed-article:8943422pubmed:day1lld:pubmed
pubmed-article:8943422pubmed:volume157lld:pubmed
pubmed-article:8943422pubmed:ownerNLMlld:pubmed
pubmed-article:8943422pubmed:authorsCompleteYlld:pubmed
pubmed-article:8943422pubmed:pagination5118-25lld:pubmed
pubmed-article:8943422pubmed:dateRevised2011-11-17lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:meshHeadingpubmed-meshheading:8943422-...lld:pubmed
pubmed-article:8943422pubmed:year1996lld:pubmed
pubmed-article:8943422pubmed:articleTitleDifferential effects of macrophage- and granulocyte-macrophage colony-stimulating factors on cytokine gene expression during rat alveolar macrophage differentiation into multinucleated giant cells (MGC): role for IL-6 in type 2 MGC formation.lld:pubmed
pubmed-article:8943422pubmed:affiliationLaboratory of Immunopharmacology, Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada.lld:pubmed
pubmed-article:8943422pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8943422pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:8943422pubmed:publicationTypeIn Vitrolld:pubmed
pubmed-article:8943422pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8943422lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8943422lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8943422lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8943422lld:pubmed