Linked Life Data

8943422

Source:http://linkedlifedata.com/resource/pubmed/id/8943422

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1996-12-27
pubmed:abstractText
Macrophage colony-stimulating factor (M-CSF) and granulocyte-macrophage (GM)-CSF stimulate the differentiation of rat alveolar macrophages (AM) into multinucleated giant cells (MGC) with distinct phenotypes (type 1 and type 2 MGC). In the present study, we analyzed the profile of cytokine gene expression induced respectively, by M-CSF and GM-CSF during rat AM differentiation using reverse transcription-PCR. Enhanced mRNA expression for IL-1alpha, TNF-alpha, and TGF-beta1 was observed 3 h after treatment with M-CSF (50 U/ml) or GM-CSF (50 U/ml). In contrast, IL-6 mRNA expression was increased by GM-CSF but not M-CSF. Kinetic analysis indicated that GM-CSF stimulated IL-6 expression early (1.5 h), with maximal effect observed at 24 h and up to 5 days thereafter. Increased mRNA levels for IL-6 were associated with higher IL-6 activity in the culture media of differentiating AM. IL-6 activity was stimulated 3 h after treatment with GM-CSF and increased with time (up to 5 days). Interestingly, addition of exogenous IL-6 (20-100 ng/ml) alone or in combination with GM-CSF to AM cultures increased slightly and selectively the formation of MGC with type 2 phenotype. Conversely, neutralization of endogenous IL-6 during AM differentiation into MGC inhibited significantly (up to 50%) the formation of type 2 MGC. These results suggest a role for IL-6 in the formation of type 2 MGC and provide some insights into the mechanisms of MGC formation and the processes that regulate it positively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5118-25
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8943422-Animals, pubmed-meshheading:8943422-Base Sequence, pubmed-meshheading:8943422-Cell Differentiation, pubmed-meshheading:8943422-Cytokines, pubmed-meshheading:8943422-DNA Primers, pubmed-meshheading:8943422-Gene Expression, pubmed-meshheading:8943422-Giant Cells, pubmed-meshheading:8943422-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:8943422-Interleukin-1, pubmed-meshheading:8943422-Interleukin-6, pubmed-meshheading:8943422-Macrophage Colony-Stimulating Factor, pubmed-meshheading:8943422-Macrophages, Alveolar, pubmed-meshheading:8943422-Male, pubmed-meshheading:8943422-Neutralization Tests, pubmed-meshheading:8943422-Polymerase Chain Reaction, pubmed-meshheading:8943422-RNA, Messenger, pubmed-meshheading:8943422-Rats, pubmed-meshheading:8943422-Rats, Wistar, pubmed-meshheading:8943422-Recombinant Proteins, pubmed-meshheading:8943422-Transforming Growth Factor beta, pubmed-meshheading:8943422-Tumor Necrosis Factor-alpha
pubmed:year
1996
pubmed:articleTitle
Differential effects of macrophage- and granulocyte-macrophage colony-stimulating factors on cytokine gene expression during rat alveolar macrophage differentiation into multinucleated giant cells (MGC): role for IL-6 in type 2 MGC formation.
pubmed:affiliation
Laboratory of Immunopharmacology, Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't