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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001721,
umls-concept:C0016677,
umls-concept:C0020964,
umls-concept:C0039194,
umls-concept:C0080194,
umls-concept:C0085358,
umls-concept:C0178719,
umls-concept:C0450254,
umls-concept:C1332714,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1517945,
umls-concept:C1704240,
umls-concept:C1706438,
umls-concept:C2698600
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pubmed:issue |
11
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pubmed:dateCreated |
1996-12-27
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pubmed:abstractText |
Normal mice readily survive a sublethal intradermal (i.d.) infection with Francisella tularensis live vaccine strain (LVS), a model intracellular bacterium, and are strongly protected against subsequent lethal challenge. However, athymic nu/nu mice, which lack mature alphabeta TCR+ T lymphocytes, succumb to i.d. infection within 30 days. Here we characterize the alphabeta T cell subpopulations necessary for both resolution of i.d. infection and generation of optimal protective immunity to LVS. BALB/cByJ mice treated with anti-CD4 or anti-CD8 Abs before i.d. infection survived and cleared bacteria, and anti-CD4- or anti-CD8-treated immune mice survived a very strong i.p. challenge of 10,000 LD50s. Among mutant mice with targeted gene disruptions (knockouts), CD4-, beta2-microglobulin-deficient (which are also CD8-), and gammadelta TCR- mice all resolved a large sublethal i.d. infection. All CD4- and beta2-microglobulin-deficient mice readily survived subsequent lethal i.p. challenge of 10,000 LD50s, even in the absence of specific IgG Abs, as did most (86%) gammadelta TCR- mice. In contrast, alphabeta TCR- mice or alphabeta + gammadelta TCR- mice died about 35 days after i.d. infection. Depletion of gammadelta+ T cells from alphabeta TCR- mice had no effect on mean time to death from i.d. LVS infection. Therefore alphabeta TCR+ cells are required for protection, but either CD4+ or CD8+ T cells are individually sufficient to resolve a large sublethal i.d. LVS infection and to protect against a maximal secondary lethal challenge. These results emphasize the remarkable plasticity of the alphabeta T cell response in protective immunity to intracellular bacteria.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/beta 2-Microglobulin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
157
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5042-8
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:8943413-Animals,
pubmed-meshheading:8943413-Antigens, CD4,
pubmed-meshheading:8943413-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8943413-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8943413-Francisella tularensis,
pubmed-meshheading:8943413-Male,
pubmed-meshheading:8943413-Mice,
pubmed-meshheading:8943413-Mice, Inbred BALB C,
pubmed-meshheading:8943413-Mice, Inbred C57BL,
pubmed-meshheading:8943413-Mice, Knockout,
pubmed-meshheading:8943413-Mice, Nude,
pubmed-meshheading:8943413-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8943413-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:8943413-T-Lymphocyte Subsets,
pubmed-meshheading:8943413-Tularemia,
pubmed-meshheading:8943413-beta 2-Microglobulin
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pubmed:year |
1996
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pubmed:articleTitle |
Loss of either CD4+ or CD8+ T cells does not affect the magnitude of protective immunity to an intracellular pathogen, Francisella tularensis strain LVS.
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pubmed:affiliation |
Laboratory of Enteric and Sexually Transmitted Diseases, Division of Bacterial Products, Center for Biologics Evaluation and Research, Rockville, MD 20852, USA.
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pubmed:publicationType |
Journal Article
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