Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1996-12-27
|
| pubmed:databankReference | |
| pubmed:abstractText |
BALB/c mice are highly susceptible to infection with the protozoan parasite Leishmania major. This susceptibility has been attributed, in part, to the expansion of parasite-specific CD4+ Th2 cells that antagonize Th1 responses and promote humoral immunity. In the present study, we have utilized sera from L. major-infected BALB/c mice to screen an L. major amastigote cDNA expression library. One of the clones detected encodes a novel Ag designated as L. major stress-inducible 1 (LmSTI1). LmSTI1 contains six copies of the tetratricopeptide consensus motif and is highly related to a family of stress-inducible proteins that is conserved from yeast to humans. Sera from L. major-infected BALB/c mice have LmSTI1-specific Ab titers in excess of 1:200,000, comprised predominantly of IgG1, IgG2A, and IgG2B isotypes. Recombinant LmSTI1 protein elicited strong proliferative responses from draining lymph node cells of L. major-infected BALB/c mice at both early (10 days) and late (28 days) stages of infection and elicited production of high levels of IFN-gamma and low levels of IL-4. In contrast, soluble leishmanial lysate elicited high levels of IL-4 and low IFN-gamma production. Thus, we have identified an Ag of Leishmania capable of eliciting a mixed cellular response that is skewed toward a Th1 phenotype in susceptible BALB/c mice with advanced infections. In addition, analyses of sera from human patients with cutaneous, visceral, and post-kala azar visceral leishmaniasis indicated that a majority of individuals from all three clinical groups mounted strong humoral responses against LmSTI1.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
157
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5034-41
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8943412-Amino Acid Sequence,
pubmed-meshheading:8943412-Animals,
pubmed-meshheading:8943412-Antibodies, Protozoan,
pubmed-meshheading:8943412-Antigens, Protozoan,
pubmed-meshheading:8943412-Base Sequence,
pubmed-meshheading:8943412-Cloning, Molecular,
pubmed-meshheading:8943412-DNA, Protozoan,
pubmed-meshheading:8943412-Disease Models, Animal,
pubmed-meshheading:8943412-Female,
pubmed-meshheading:8943412-Genes, Protozoan,
pubmed-meshheading:8943412-Humans,
pubmed-meshheading:8943412-Interferon-gamma,
pubmed-meshheading:8943412-Interleukin-4,
pubmed-meshheading:8943412-Leishmania major,
pubmed-meshheading:8943412-Leishmaniasis, Cutaneous,
pubmed-meshheading:8943412-Mice,
pubmed-meshheading:8943412-Mice, Inbred BALB C,
pubmed-meshheading:8943412-Molecular Sequence Data,
pubmed-meshheading:8943412-T-Lymphocytes
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Molecular cloning of a novel protein antigen of Leishmania major that elicits a potent immune response in experimental murine leishmaniasis.
|
| pubmed:affiliation |
Infectious Disease Research Institute, Seattle, WA 98104, USA.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|