Linked Life Data

8941648

Source:http://linkedlifedata.com/resource/pubmed/id/8941648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-1-23
pubmed:abstractText
Group B Streptococcus (GBS) is an important perinatal pathogen. Because transplacentally acquired maternal antibodies to the GBS capsular polysaccharides (CPS) confer protection, prevention of infant disease may be possible after immunization of women. Unfortunately, the purified CPS of GBS are only variably immunogenic in adults; therefore to enhance immunogenicity we have designed and developed a CPS-protein conjugate vaccine. The lability of a conformationally dependent epitope on the III CPS containing a critical sialic acid residue was important to consider in vaccine design. 100 women were randomized to receive GBS type III CPS-tetanus toxoid conjugate (III-TT) vaccine at one of three doses; unconjugated GBS type III CPS; or saline. Serum samples were obtained before immunization and 2, 4, 8, and 26 wk thereafter, and specific antibody to type III CPS was measured. Vaccines were well tolerated. In sera from recipients of the highest dose of III-TT, CPS-specific IgG levels rose from a geometric mean of 0.09 microg/ml before immunization to 4.53 microg/ml 8 wk later, whereas levels in recipients of unconjugated type III CPS rose from 0.21 microg/ml to 1.41 microg/ml. Lower doses resulted in lower antibody levels. A > or = 4-fold rise in antibody concentration was achieved in 90% of recipients of III-TT compared with 50% of those that received III CPS (P = 0.0015). Antibodies evoked by the conjugate vaccine recognized a conformationally dependent epitope of the III-CPS, promoted opsonophagocytosis and killing of GBS, and, after maternal immunization, protected neonatal mice from lethal challenge with type III GBS. We conclude that directed coupling of type III GBS polysaccharide to a carrier protein yielded a conjugate vaccine with preserved expression of a highly labile conformational epitope involving sialic acid and enhanced immunogenicity compared with uncoupled CPS.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-1097573, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-1398913, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-1500748, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-1729272, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-2243123, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-2471773, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-2675485, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-3050524, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-3480537, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-3898306, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-4556115, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-6170311, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7033911, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7639440, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7639441, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7659485, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-768760, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7706814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7713185, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7798688, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-7970951, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-8371444, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-8406875, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-8502269, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-8537651, http://linkedlifedata.com/resource/pubmed/commentcorrection/8941648-856869
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2308-14
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:8941648-Adolescent, pubmed-meshheading:8941648-Adult, pubmed-meshheading:8941648-Animals, pubmed-meshheading:8941648-Antibodies, Bacterial, pubmed-meshheading:8941648-Antibody Affinity, pubmed-meshheading:8941648-Antibody Specificity, pubmed-meshheading:8941648-Cytotoxicity, Immunologic, pubmed-meshheading:8941648-Epitopes, pubmed-meshheading:8941648-Female, pubmed-meshheading:8941648-Humans, pubmed-meshheading:8941648-Immunoglobulin A, pubmed-meshheading:8941648-Immunoglobulin G, pubmed-meshheading:8941648-Immunoglobulin M, pubmed-meshheading:8941648-Mice, pubmed-meshheading:8941648-N-Acetylneuraminic Acid, pubmed-meshheading:8941648-Opsonin Proteins, pubmed-meshheading:8941648-Phagocytosis, pubmed-meshheading:8941648-Polysaccharides, Bacterial, pubmed-meshheading:8941648-Streptococcal Infections, pubmed-meshheading:8941648-Streptococcus agalactiae, pubmed-meshheading:8941648-Vaccines, pubmed-meshheading:8941648-Vaccines, Conjugate
pubmed:year
1996
pubmed:articleTitle
Immune response to type III group B streptococcal polysaccharide-tetanus toxoid conjugate vaccine.
pubmed:affiliation
Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. dkasper@warren.med.harvard.edu
pubmed:publicationType
Journal Article, Clinical Trial, Research Support, U.S. Gov't, P.H.S., Randomized Controlled Trial, Research Support, Non-U.S. Gov't