Linked Life Data

8941338

Source:http://linkedlifedata.com/resource/pubmed/id/8941338

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-1-6
pubmed:abstractText
The reactivity of rabbit reticulocyte 15-lipoxygenase (15-LOX) with phosphatidylcholine (PC) possessing linoleic acid (LA-PC), arachidonic acid (AA-PC), or docosahexaenoic acid (DHA-PC) was investigated by measuring oxygen uptake in the suspension of large unilamellar liposomes (LUV). The 15-LOX reaction with PC LUV was found to follow Michaelis-Menten kinetics. The apparent values of the Michaelis constants (K(m)) of LUV of LA-PC, AA-PC, and DHA-PC were nearly the same (1.0-1.2 mM). However, the maximum velocity (Vmax) of DHA-PC was obviously two to three times lower than those of LA-PC and AA-PC. On the other hand, the oxidizability of PC per bis-allylic H of unsaturated fatty acids in the free radical chain reaction of LUV decreased with increasing degree of unsaturation, that is, LA-PC > AA-PC > DHA-PC. These results suggested that the oxidizability of the DHA moiety incorporated into biomembranes is not necessarily high in lipoxygenase reaction as in free radical chain reaction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
228
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
675-82
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Reactivity of mammalian 15-lipoxygenase with phospholipids in large unilamellar liposomes.
pubmed:affiliation
Department of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Japan.
pubmed:publicationType
Journal Article