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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-2-28
|
| pubmed:abstractText |
To provide an objective assessment of the comparative utility of fluorescence- and peroxidase-based immunohistochemistry and electron microscopy, an observer blinded study was conducted under realistic study conditions utilizing a large sampling of poorly differentiated pediatric round cell tumors. Working independently, using a single ancillary technique of particular expertise, each of three investigators attempted to render a specific diagnosis with regard to 50 diagnostically challenging tumors. The results were compared against the subsequent "file diagnosis" established by consensus with all relevant information made available. A grading scheme was applied wherein points were awarded based on the accuracy and confidence of diagnosis. A comparative efficiency rating, expressed as a percentage, was formulated by dividing the number of points awarded each technique by the total number of points theoretically available. Electron microscopy proved superior overall, with an efficiency rating of 89%. Immunoperoxidase and immunofluorescence studies yielded efficiency ratings of 71 and 61%, respectively. Used in combination, the techniques achieved an efficiency rating of 95%. Application of these ancillary techniques resulted in a revision of the provisional diagnosis in 11 of 50 cases, and left only two cases without a firm specific diagnosis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0191-3123
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
507-17
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8940758-Adolescent,
pubmed-meshheading:8940758-Adult,
pubmed-meshheading:8940758-Child,
pubmed-meshheading:8940758-Child, Preschool,
pubmed-meshheading:8940758-Female,
pubmed-meshheading:8940758-Humans,
pubmed-meshheading:8940758-Immunoenzyme Techniques,
pubmed-meshheading:8940758-Infant,
pubmed-meshheading:8940758-Lymphoma,
pubmed-meshheading:8940758-Male,
pubmed-meshheading:8940758-Mesenchymoma,
pubmed-meshheading:8940758-Microscopy, Electron,
pubmed-meshheading:8940758-Neuroblastoma,
pubmed-meshheading:8940758-Neuroectodermal Tumors, Primitive, Peripheral,
pubmed-meshheading:8940758-Pathology, Surgical,
pubmed-meshheading:8940758-Rhabdoid Tumor,
pubmed-meshheading:8940758-Rhabdomyosarcoma,
pubmed-meshheading:8940758-Sarcoma, Ewing,
pubmed-meshheading:8940758-Sarcoma, Small Cell,
pubmed-meshheading:8940758-Wilms Tumor
|
| pubmed:articleTitle |
Appraisal of the comparative utility of immunohistochemistry and electron microscopy in the diagnosis of childhood round cell tumors.
|
| pubmed:affiliation |
Department of Pathology, Children's Hospital Denver, CO 80218, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|