Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-2-4
pubmed:databankReference
pubmed:abstractText
Hemicellulose is a major component of primary plant cell walls. Many of the glycosyl residues found in hemicellulose are derived from the sugar precursor UDP-glucuronic acid, which can be converted into UDP-arabinose, UDP-apiose, UDP-galacturonic acid, and UDP-xylose. The enzyme controlling the biosynthesis of UDP-glucuronic acid, UDP-glucose dehydrogenase (EC 1.1.1.22), was cloned from soybean (Glycine max [L.] Merr.) by an antibody screening procedure. This enzyme is surprisingly homologous to the bovine sequence, which is the only other eukaryotic UDP-glucose dehydrogenase sequence known. The characteristic motifs of the enzyme, the catalytic center, a NAD-binding site, and two proline residues for main chain bends, are conserved within the prokaryotic and eukaryotic sequences. The soybean full-length cDNA clone encodes a protein of 480 amino acids with a predicted size of 52.9 kD. The enzyme is highly expressed in young roots, but lower expression levels were observed in expanding tissues of the epicotyl and in young leaves. The expression pattern of the enzyme in different developmental stages strengthens the argument that UDP-glucose dehydrogenase is a key regulator for the availability of hemicellulose precursors.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-11607542, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-16658622, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-16666104, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-17739625, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-192218, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-2547247, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-4342114, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-6326095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-6896145, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-7846151, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-7920253, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-8088456, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-8346556, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-8540790, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-8546701, http://linkedlifedata.com/resource/pubmed/commentcorrection/8938413-8718627
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1127-34
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed-meshheading:8938413-Amino Acid Sequence, pubmed-meshheading:8938413-Animals, pubmed-meshheading:8938413-Arabidopsis, pubmed-meshheading:8938413-Base Sequence, pubmed-meshheading:8938413-Cattle, pubmed-meshheading:8938413-Cloning, Molecular, pubmed-meshheading:8938413-Databases, Factual, pubmed-meshheading:8938413-Gene Expression Regulation, Developmental, pubmed-meshheading:8938413-Gene Expression Regulation, Enzymologic, pubmed-meshheading:8938413-Gene Expression Regulation, Plant, pubmed-meshheading:8938413-Genes, Plant, pubmed-meshheading:8938413-Molecular Sequence Data, pubmed-meshheading:8938413-Polysaccharides, pubmed-meshheading:8938413-Recombinant Proteins, pubmed-meshheading:8938413-Sequence Homology, Amino Acid, pubmed-meshheading:8938413-Soybeans, pubmed-meshheading:8938413-Uridine Diphosphate Glucose Dehydrogenase
pubmed:year
1996
pubmed:articleTitle
Cloning of an enzyme that synthesizes a key nucleotide-sugar precursor of hemicellulose biosynthesis from soybean: UDP-glucose dehydrogenase.
pubmed:affiliation
Universität Kaiserslautern, Germany. tenhaken@rhrk.uni-kl.de
pubmed:publicationType
Journal Article, Comparative Study