Linked Life Data

8936051

Source:http://linkedlifedata.com/resource/pubmed/id/8936051

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-20
pubmed:abstractText
We have determined the cDNA sequence of a member of the channel catfish heat shock protein 70 (CF Hsp70) family. This protein presumably functions as a molecular chaperone, as is characteristic of this family in other species. Channel catfish peripheral blood leukocytes exhibit a classical heat shock response, in that heat shock (37 degrees C) induces the expression of heat shock genes that are quiescent at normal temperatures (23 degrees C). It was observed that pre-existing synthesis of certain other molecules was suppressed (as evidenced by decreases in actin RNA upon heat shock). Similar trends were observed in mRNA expression for CF Hsp70 in two catfish non-leukocyte cell lines, channel catfish ovary and F59. However, three leukocyte cell lines constitutively expressed high levels of CF Hsp70 mRNA at optimal culture temperature (27 degrees C), whereas heat shock (37 degrees C) elicited only a modest induction of CF Hsp70 expression. Furthermore, continued investigation is warranted to determine whether the apparent upregulation of CF Hsp70 mRNA expression in the catfish long-term leukocyte cell lines is involved in the seemingly immortal phenotype of these cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Identification and characterization of a heat shock protein 70 family member in channel catfish (Ictalurus punctatus).
pubmed:affiliation
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.